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Discovery of a novel Tymoviridae-like virus in mosquitoes from Mexico

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Abstract

A novel Tymoviridae-like virus, designated Ek Balam virus, was isolated from male Culex quinquefasciatus mosquitoes collected in Yucatan, Mexico. The genome was fully sequenced and shown to have no more than 69% nt sequence identity to its closest known relative. Mosquito cells were permissive to Ek Balam virus replication, but mammalian and avian cells were refractory, suggesting that vertebrates are not involved in the maintenance of the virus in nature.

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Acknowledgements

Mosquito collections were made possible by grant 5R21AI067281 from the National Institutes of Health. The metagenomics analysis and in vitro host range experiments were supported by an intramural grant from Iowa State University. A.E.F. and C.T. are supported by a Wellcome Trust grant [106207] and a European Research Council grant [646891] to A.E.F. The authors thank Kristina Larsen and Sean Johnston for technical assistance.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 2116 Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA

    Jermilia Charles, Chandra S. Tangudu, Stefanie L. Hurt & Bradley J. Blitvich

  2. Department of Pathology, University of Cambridge, Cambridge, UK

    Charlotte Tumescheit & Andrew E. Firth

  3. Laboratorio de Arbovirologia, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico

    Julian E. Garcia-Rejon & Carlos Machain-Williams

Authors
  1. Jermilia Charles
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  2. Chandra S. Tangudu
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  3. Stefanie L. Hurt
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  4. Charlotte Tumescheit
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  5. Andrew E. Firth
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  6. Julian E. Garcia-Rejon
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  7. Carlos Machain-Williams
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  8. Bradley J. Blitvich
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Corresponding author

Correspondence to Bradley J. Blitvich.

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The authors declare that they have no conflict of interest.

Ethical approval

The research reported here did not involve the use of human subjects or vertebrate animals.

Ethical statement

This study represents original work that has not been submitted to any other journal for publication. The authors have no conflict of interest to declare. No human or animal ethics approval was required for the completion of this study.

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Handling Editor: T. K. Frey.

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Charles, J., Tangudu, C.S., Hurt, S.L. et al. Discovery of a novel Tymoviridae-like virus in mosquitoes from Mexico. Arch Virol 164, 649–652 (2019). https://doi.org/10.1007/s00705-018-4098-x

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  • DOI: https://doi.org/10.1007/s00705-018-4098-x

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