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Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles

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Abstract

P70 is a Pinot Noir grapevine accession that displays strong leafroll disease symptoms. A high-throughput sequencing (HTS)-based analysis established that P70 was mixed-infected by two variants of grapevine leafroll-associated virus 1 (GLRaV-1, genus Ampelovirus) and one of grapevine virus A (GVA, genus Vitivirus) as well as by two viroids (hop stunt viroid [HSVd] and grapevine yellow speckle viroid 1 [GYSVd1]) and four variants of grapevine rupestris stem pitting-associated virus (GRSPaV). Immunogold labelling using gold particles of two different diameters revealed the existence of ‘hybrid’ particles labelled at one end as GLRaV-1, with the rest labelled as GVA. In this work, we suggest that immunogold labelling can provide information about the biology of the viruses, going deeper than just genomic information provided by HTS, from which no recombinant or ‘chimeric’ GLRaV-1/GVA sequences had been identified in the dataset. Our observations suggest an unknown interaction between members of two different viral species that are often encountered together in a single grapevine, highlighting potential consequences in the vector biology and epidemiology of leafroll and rugose-wood diseases.

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Acknowledgements

This work was supported by the Institut National de la Recherche Agronomique (INRA) and partially supported by the Agence Nationale pour la Recherche, ANR Vinobodies contract: ANR-14-CE19-0018-02. JMH was funded by the “Metagenome” (28000225) project gathering three French professional committees for viticulture (Interprofessional Committee of Champagne Wine, CIVC, Epernay; Interprofessional Office of Burgundy Wines, BIVB, Beaune and Interprofessional Committee of Wines from Alsace, CIVA, Colmar) and Moët & Chandon. The authors acknowledge Jacky Misbach and the greenhouse team for technical support, and Lionel Ley and the members of the experimental unit of INRA-Colmar for the production of plants.

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Author notes

  1. Antoine Alliaume and Catherine Reinbold contributed equally to the work.

Authors and Affiliations

  1. SVQV, Université de Strasbourg, INRA, 68000, Colmar, France

    Antoine Alliaume, Catherine Reinbold, Monique Beuve, Jean-Michel Hily, Olivier Lemaire & Etienne Herrbach

  2. Institut de Biologie Moléculaire des Plantes, CNRS UPR2357, Université de Strasbourg, 67084, Strasbourg, France

    Mathieu Erhardt

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  1. Antoine Alliaume
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  2. Catherine Reinbold
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  3. Mathieu Erhardt
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  4. Monique Beuve
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  5. Jean-Michel Hily
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  6. Olivier Lemaire
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  7. Etienne Herrbach
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Correspondence to Olivier Lemaire.

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Handling Editor: F. Murilo Zerbini.

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Alliaume, A., Reinbold, C., Erhardt, M. et al. Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles. Arch Virol 163, 3149–3154 (2018). https://doi.org/10.1007/s00705-018-3995-3

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