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Molecular characterization of a novel double-stranded RNA mycovirus of Trichoderma asperellum strain JLM45-3

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Abstract

In this study, we describe a novel mycovirus isolated from Trichoderma asperellum, which was designated Trichoderma asperellum dsRNA Virus 1 (TaRV1). The sequence analysis revealed that TaRV1 has two discontinuous open reading frames (ORF), ORF1 and ORF2. A hypothetical protein and an RNA-dependent RNA polymerase are encoded by ORF1 and ORF2, respectively. Phylogenetic analysis based on RdRp sequences clearly places TaRV1 in a taxonomically unassigned dsRNA mycovirus group.

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References

  1. Nuss DL, Koltin Y (1990) Significance of dsRNA genetic elements in plant pathogenic fungi. Annu Rev Phytopathol 28:37–58

    Article  CAS  Google Scholar 

  2. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    Article  CAS  Google Scholar 

  3. Ghabrial SA, Suzuki N (2008) Fungal viruses. Encycl Virol 2:284–291

    Article  Google Scholar 

  4. Wickner RB, Ghabrial SA, Nibert ML, Patterson JL, Wang CC (2011) Family Totiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, 9th ICTV -report. Elsevier Inc., Amsterdam, pp 639–650

    Google Scholar 

  5. Lee SH, Suk-Hyun Y, Jeesun C, Dae-Hyuk K (2017) Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028. Arch Virol 162(4):1073–1077

    Article  CAS  Google Scholar 

  6. Wang L, Zhang J, Zhang H, Qui D, Guo L (2016) Two novel relative double-stranded RNA mycoviruses infecting Fusarium poae strain SX63. Int J Mol Sci 17:641

    Article  Google Scholar 

  7. Marvelli RA, Hobbs HA, Li S, McCoppin NK, Domier LL, Hartman GL, Eastburn DM (2014) Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence. Arch Virol 159:349–352

    Article  CAS  Google Scholar 

  8. Yu L, Sang W, Wu MD, Zhang J, Yang L, Zhou YJ, Chen WD, Li GQ (2015) Novel hypovirulence-associated RNA mycovirus in the plant-pathogenic fungus Botrytis cinerea: molecular and biological characterization. Appl Environ Microbiol 81:2299–2310

    Article  CAS  Google Scholar 

  9. Sticher L, Mauch-Mani B, Métraux JP (1997) Systemic acquired resistance. Annu Rev Phytopathol 35:235–270

    Article  CAS  Google Scholar 

  10. Ulhoa CJ, Peberdy JF (1992) Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme Microb Technol 14:236–240

    Article  CAS  Google Scholar 

  11. Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma spp.—opportunistic avirulent plant symbionts. Nat Microbiol Rev 2:43–56

    Article  CAS  Google Scholar 

  12. Kim DH, Yun SH, Lee SH, So KK, Kim JM (2016) Incidence of diverse dsRNA mycoviruses in Trichoderma spp. causing green mold disease of shiitake Lentinula edodes. FEMS Microbiol Lett. https://doi.org/10.1093/femsle/fnw220

    Article  PubMed  Google Scholar 

  13. Morris T, Dodds J (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  14. Froussard P (1993) rPCR: a powerful tool for random amplification of whole RNA sequences. Genome Res 2(3):185–190

    Article  CAS  Google Scholar 

  15. Lambden PR, Cooke SJ, Caul EO, Clarke IN (1992) Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66:1817–1822

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  CAS  Google Scholar 

  17. Donaire L, Ayllon MA (2017) Deep sequencing of mycovirus-derived small RNAs from Botrytis species. Mol Plant Pathol 18(8):1127–1137

    Article  CAS  Google Scholar 

  18. Yu L, SangW WuMD, Zhang J, Yang L, Zhou YJ, Chen WD, Li GQ (2015) Novel hypovirulence-associated RNA mycovirus in the plant-pathogenic fungus Botrytis cinerea: molecular and biological characterization. Appl Environ Microbiol 18(7):2299–2310

    Article  Google Scholar 

Download references

Funding

This study was funded by Grants from the National Natural Science Foundation of China (31760015); the Science and Technology Foundation of Guizhou Province([2017]1153); Joint Foundation of Collaboration Project between Scientific and Technological Bureau of Guizhou Province and Universities of Guizhou Province (LH[2015]7322); the Bureau of Science and Technology of Guiyang ([2017]5-24); the Doctoral Cultivating Fund of Guizhou Medical University (J[2014]025); Engineering Research Center of Medical Biotechnology, Guizhou Medical University(2016002); Project of Guizhou Provincial Education Department for Youth Science and Technology Talent Development (KY[2016]145).

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Author notes

  1. Tingting Zhang and Xiangxing Zeng contributed equally to this work.

Authors and Affiliations

  1. Engineering Research Center of Medical Biotechnology, Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, 550000, Guizhou, People’s Republic of China

    Tingting Zhang, Xiangxing Zeng, Xiaoyao Cai, Hongmei Liu & Zhu Zeng

  2. Immune Cells and Antibody Engineering Research Center of Guizhou Province, Cell and Gene Engineering Innovative Research Groups of Guizhou Province, Guizhou Medical University, Guiyang, 550000, Guizhou, People’s Republic of China

    Tingting Zhang, Xiangxing Zeng, Xiaoyao Cai, Hongmei Liu & Zhu Zeng

  3. College of Biology and Engineering, Guizhou Medical University, Guiyang, 550000, Guizhou, People’s Republic of China

    Tingting Zhang, Xiangxing Zeng, Xiaoyao Cai, Hongmei Liu & Zhu Zeng

Authors
  1. Tingting Zhang
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  2. Xiangxing Zeng
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  3. Xiaoyao Cai
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  4. Hongmei Liu
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  5. Zhu Zeng
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Corresponding author

Correspondence to Tingting Zhang.

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The authors declare no conflict of interest.

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This article does not contain any studies with animals or human participants performed by any of the authors.

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Handling Editor: Massimo Turina.

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Supplementary Figure S1

Predicted secondary structure in TaRV1 (PDF 14 kb)

Supplementary Figure S2

Multiple alignments of the amino acid sequences of conserved motifs in the encoded RNA-dependent RNA polymerase (RdRp), the S7 domain in ORF2, for TaRV1 and related viruses. See legend to Figure 2 for abbreviations of the virus names (EMF 988 kb)

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Zhang, T., Zeng, X., Cai, X. et al. Molecular characterization of a novel double-stranded RNA mycovirus of Trichoderma asperellum strain JLM45-3. Arch Virol 163, 3433–3437 (2018). https://doi.org/10.1007/s00705-018-3988-2

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  • DOI: https://doi.org/10.1007/s00705-018-3988-2

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