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Genomic analysis of a novel isolate Heliothis virescens ascovirus 3i (HvAV-3i) and identification of ascoviral repeat ORFs (aros)

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Abstract

Ascoviruses are circular double-stranded DNA viruses that infect insects. Herein we sequenced and analyzed the genome of the previously unrecorded ascovirus isolate Heliothis virescens ascovirus 3i (HvAV-3i). The genome size is 185,650 bp with 181 hypothetical open reading frames (ORFs). Additionally, definition based on ascovirus repeated ORFs (aros) is proposed; whereby the 29 aros from all sequenced Ascoviridae genomes are divided into six distinct groups. The topological relationship among the isolates of Heliothis virescens ascovirus 3a is (HvAV-3f, {HvAV-3h, [HvAV-3e, (HvAV-3g, HvAV-3i)]}) with every clade well supported by a Bayesian posterior probability of 1.00 and a Bootstrap value of 100%.

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Acknowledgements

The authors would like to thank Jue Hu, Hai-Zhou Liu and Lei Zhang for their help in virus amplification and data analysis. The authors would also like to acknowledge Bi-Chao Xu from the Core Facility and Technical Support of Wuhan Institute of Virology for the technical assistance in SEM and TEM analysis. We thank Dr. Cecil L. Smith (University of Georgia, U.S.A.) for editing and revising the English language.

Funding

This study was partially supported by the National Natural Science Foundation of China (No. 31371995) and STS Project of the Chinese Academy of Sciences (No. KFJ-SW-STS143-3).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Zi-Shu Chen, Xing Wang & Guo-Hua Huang

  2. Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Zi-Shu Chen, Xing Wang & Guo-Hua Huang

  3. School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China

    Dian-Hai Hou

  4. Department of Microbiology, 56 Pearson Hall, Miami University, Oxford, 45056, Ohio, USA

    Xiao-Wen Cheng

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  1. Zi-Shu Chen
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Correspondence to Guo-Hua Huang.

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The authors have declared that no competing interests exist. This article does not contain any studies with human or animal subjects performed by any of the authors.

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Chen, ZS., Hou, DH., Cheng, XW. et al. Genomic analysis of a novel isolate Heliothis virescens ascovirus 3i (HvAV-3i) and identification of ascoviral repeat ORFs (aros). Arch Virol 163, 2849–2853 (2018). https://doi.org/10.1007/s00705-018-3899-2

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  • DOI: https://doi.org/10.1007/s00705-018-3899-2

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