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Sequencing, genome analysis and host range of a novel Ralstonia phage, RsoP1EGY, isolated in Egypt

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Abstract

A novel Ralstonia phage was isolated from soil in Egypt. It was designated Ralstonia phage RsoP1EGY using our phage identifier naming approach to reflect the phage’s bacterial host species, characteristics and origin. When tested, this phage specifically infected only race 3 biovar 2 phylotype IIB sequevar 1, and not non-race 3 biovar 2 strains of Ralstonia solanacearum. The phage has an icosahedral capsid of 60 ± 5 nm in diameter with a short tail of 15 ± 5 nm in length, typical of a podovirus. The genome of RsoP1EGY is 41,297 bp in size, containing 50 open reading frames, with no significant sequence identity to any other reported R. solanacearum or non-Ralstonia phages, except to the recently deposited but unreported and unclassified Ralstonia phage DU_RP_I. RsoP1EGY is the first sequenced and characterized R. solanacearum phage isolated in Egypt.

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Authors and Affiliations

  1. Floral and Nursery Plants Research Unit, United States National Arboretum, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA

    Abdelmonim Ali Ahmad, Hardian Susilo Addy & Qi Huang

  2. Department of Plant Pathology, Faculty of Agriculture, Minia University, El-minia, Egypt

    Abdelmonim Ali Ahmad

  3. Bacterial Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt

    Kamel M. Elhalag & Ahmed S. Hussien

  4. Potato Brown Rot Project, Ministry of Agriculture, Dokki, Giza, Egypt

    Kamel M. Elhalag

  5. Department of Plant Protection, Faculty of Agriculture, University of Jember, Jember, Indonesia

    Hardian Susilo Addy

  6. Botany Department, Faculty of Science, Benha University, Benha, Qalyubiya Governorate, Egypt

    Mohamed A. Nasr-Eldin

  7. Central Administration of Plant Quarantine, Dokki, Giza, Egypt

    Ahmed S. Hussien

Authors
  1. Abdelmonim Ali Ahmad
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  2. Kamel M. Elhalag
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  3. Hardian Susilo Addy
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  4. Mohamed A. Nasr-Eldin
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  5. Ahmed S. Hussien
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  6. Qi Huang
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Correspondence to Qi Huang.

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All authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Johannes Wittmann.

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Ahmad, A.A., Elhalag, K.M., Addy, H.S. et al. Sequencing, genome analysis and host range of a novel Ralstonia phage, RsoP1EGY, isolated in Egypt. Arch Virol 163, 2271–2274 (2018). https://doi.org/10.1007/s00705-018-3844-4

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  • DOI: https://doi.org/10.1007/s00705-018-3844-4

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