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Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone

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Abstract

The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.

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Acknowledgements

The authors are grateful to Dr. Ben Dugdale, Queensland University of Technology, for providing the pOPT-NXT vector for cloning purposes. DK is the recipient of an Australia Awards Scholarship.

Data availability

Sequences described in this paper are available under GenBank accession numbers MG017318-MG017320.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Queensland University of Technology (QUT), Brisbane, 4001, Australia

    Dawit B. Kidanemariam, Amit C. Sukal, James L. Dale, Robert M. Harding & Anthony P. James

  2. National Agricultural Biotechnology Research Center, Ethiopian Institute of Agricultural Research, P.O. Box 2003, Addis Ababa, Ethiopia

    Dawit B. Kidanemariam

  3. Department of Agriculture and Fisheries, Eco-sciences Precinct, Dutton Park, Brisbane, 4102, Australia

    Kathy Crew

  4. Grahame Jackson, 24 Alt St, Queens Park, NSW, 2022, Australia

    Grahame V. H. Jackson

  5. Department of Biotechnology, Addis Ababa Science and Technology University, P.O. Box 16417, Addis Ababa, Ethiopia

    Adane D. Abraham

Authors
  1. Dawit B. Kidanemariam
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  2. Amit C. Sukal
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  3. Kathy Crew
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  4. Grahame V. H. Jackson
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  5. Adane D. Abraham
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  6. James L. Dale
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  7. Robert M. Harding
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  8. Anthony P. James
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Corresponding author

Correspondence to Anthony P. James.

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The authors declare they have no conflict of interest.

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This article does not contain any work conducted on animals or human participants.

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Handling Editor: Sead Sabanadzovic.

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Kidanemariam, D.B., Sukal, A.C., Crew, K. et al. Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone. Arch Virol 163, 1677–1681 (2018). https://doi.org/10.1007/s00705-018-3783-0

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  • DOI: https://doi.org/10.1007/s00705-018-3783-0

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