Skip to main content
SpringerLink
Log in

Complete genome sequence of SRT8, a novel T1-like Escherichia coli bacteriophage

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In this study, an Escherichia coli virulent phage, SRT8, was isolated from sewage sludge samples collected from Jinan, Shandong Province, China. The genome of phage SRT8 consists of 49,579 bp with 47.83% G+C content. The phage genome contains 84 putative protein-coding genes, and no rRNA or tRNA genes. Comparative genomics analysis showed that the E. coli phage SRT8 is a member of a new species and belongs to the subfamily Tunavirinae, which includes T1-like phages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Sulakvelidze A (2005) Phage therapy: an attractive option for dealing with antibiotic-resistant bacterial infections. Drug Discov Today 10(12):807–809

    Article  PubMed  Google Scholar 

  2. Hagens S, Loessner MJ (2010) Bacteriophage for biocontrol of foodborne pathogens: calculations and considerations. Curr Pharm Biotechnol 11(1):58–68

    Article  PubMed  CAS  Google Scholar 

  3. Withey S, Cartmell E, Avery LM, Stephenson T (2005) Bacteriophages—potential for application in wastewater treatment processes. Sci Total Environ 339(1–3):1–18

    Article  PubMed  CAS  Google Scholar 

  4. Ishii S, Sadowsky MJ (2008) Escherichia coli in the environment: implications for water quality and human health. Microbes Environ 23(2):101–108

    Article  PubMed  Google Scholar 

  5. Amézquitalópez BA, Quiñones B, Sotobeltrán M, Lee BG, Yambao JC, Lugomelchor OY, Chaidez C (2016) Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico. Antimicrob Resist Infect Control 5(1):1–6

    Article  Google Scholar 

  6. Fan X, Yan J, Xie L, Zeng L, Young RF, Xie J (2015) Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil. Gene 561(1):45–53

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Delcher AL, Bratke KA, Powers EC, Salzberg SL (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23(6):673–679

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T, Ussery DW (2007) RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 35(9):3100–3108

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. Schattner P, Brooks AN, Lowe TM (2005) The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res 33:W686–W689 (web server issue)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647–1649

    Article  PubMed  PubMed Central  Google Scholar 

  11. Niu YD, McAllister TA, Nash JH, Kropinski AM, Stanford K (2014) Four Escherichia coli O157:H7 phages: a new bacteriophage genus and taxonomic classification of T1-like phages. PLoS One 9(6):e100426

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Funding

This study was funded by National Natural Science Foundation (Grant numbers 31600148, 31100088, and 31600066), Shandong Excellent Young Scientist Award Fund (Grant number BS2014YY031), Shandong Provincial Natural Science Foundation (ZR2016CB20), Shandong Province Science and Technology Development Plan (Grant number 2013GSF12006); Foundation of University of Jinan (Grant numbers XBS1519, and XKY1633).

Author information

Author notes

  1. Kaili Zhao, Shukai Song and Zhipeng Zhao contributed equally to this work.

Authors and Affiliations

  1. School of Biological Science and Technology, University of Jinan, Jinan, 250022, Shandong, China

    Kaili Zhao, Shukai Song, Zhipeng Zhao, Pengfei Gu, Xiangyu Fan & Qiang Li

Authors
  1. Kaili Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shukai Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhipeng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pengfei Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiangyu Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiangyu Fan or Qiang Li.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: T. K. Frey.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, K., Song, S., Zhao, Z. et al. Complete genome sequence of SRT8, a novel T1-like Escherichia coli bacteriophage. Arch Virol 163, 1705–1708 (2018). https://doi.org/10.1007/s00705-018-3778-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-018-3778-x

Search

Navigation