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Maize Iranian mosaic virus shows a descending transcript accumulation order in plant and insect hosts

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Abstract

Maize Iranian mosaic virus (MIMV) is a distinct member of the genus Nucleorhabdovirus. In this study, expression of all MIMV genes in maize for four weeks after inoculation and in inoculative planthoppers was examined using a quantitative RT-PCR (RT-qPCR) assay. Accumulation of MIMV P, gene 3, M, G and L transcripts relative to N transcripts was measured and normalized to 18S rRNA in maize plants and to the ribosomal protein S13 gene (RPS13) in planthoppers using the comparative CT method. In plants, higher levels of MIMV N transcripts were found relative to other transcripts, while MIMV L transcripts were at the lowest levels. The highest accumulation of MIMV transcripts was found at 14 days postinoculation (dpi). At 21 dpi, we found the lowest transcript levels for all genes, which increased again at 28 dpi, although in lower amounts than at 14 dpi. In Laodelphax striatellus, MIMV M, G and L transcripts accumulated at lower levels than other transcripts. The gene 3 transcript level was high in both plants and planthoppers. Our results showed that transcript accumulation for the MIMV genes was similar in both hosts and followed the pattern of sequential transcriptional attenuation from the 3ˊ to the 5ˊ end of the genome, similar to vertebrate rhabdoviruses. These results indicate that the regulation of virus gene transcription for this plant-infecting rhabdovirus is similar to that of some vertebrate-infecting rhabdoviruses.

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Funding

This study was supported by Research and Technology Affairs of Isfahan University of Technology.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Mozhgan Hortamani & Amir Massah

  2. Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, 71441-65186, Iran

    Keramat Izadpanah

Authors
  1. Mozhgan Hortamani
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  2. Amir Massah
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  3. Keramat Izadpanah
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Correspondence to Amir Massah.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Handling Editor: Ralf Georg Dietzgen.

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Hortamani, M., Massah, A. & Izadpanah, K. Maize Iranian mosaic virus shows a descending transcript accumulation order in plant and insect hosts. Arch Virol 163, 887–893 (2018). https://doi.org/10.1007/s00705-017-3680-y

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  • DOI: https://doi.org/10.1007/s00705-017-3680-y

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