Skip to main content
Log in

Molecular characterization of a genetically divergent equine pegivirus strain identified in China

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

A Correction to this article was published on 23 November 2017

This article has been updated

Abstract

Equine pegivirus (EPgV) is a newly discovered equine virus, which is taxonomically classified in the Pegivirus genus of the Flaviviridae family. Until now, only the complete genome sequence of the first reported EPgV strain, from the USA (strain name: C0035) is available on online databases. Considering this, horse serum samples were collected from horses in China and screened for EPgV RNA by RT-PCR. One EPgV strain, LW/2016, was obtained and its near-complete genome sequence was acquired by standard PCR. Further analysis of its nucleotide sequence indicates LW/2016 is genetically divergent from C0035, with a nucleotide identity of 89.02%. These two viruses clustered into two independent branches following phylogenetic analysis of on the NS3 and NS5B genes. To our knowledge, this is the first report of genetic divergence in the EPgV genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

Change history

  • 23 November 2017

    Unfortunately, the corresponding author name was incorrectly published in the original article and the same is corrected here. The original article was corrected.

References

  1. Smith DB, Becher P, Bukh J, Gould EA, Meyers G, Monath T, Muerhoff AS, Pletnev A, Rico-Hesse R, Stapleton JT, Simmonds P (2016) Proposed update to the taxonomy of the genera Hepacivirus and Pegivirus within the Flaviviridae family. J Gen Virol 97(11):2894–2907. doi:10.1099/jgv.0.000612

    Article  CAS  PubMed  Google Scholar 

  2. Pfaender S, Brown RJ, Pietschmann T, Steinmann E (2014) Natural reservoirs for homologs of hepatitis C virus. Emerg Microbes Infect 3(3):e21. doi:10.1038/emi.2014.19

    Article  PubMed  PubMed Central  Google Scholar 

  3. Adams MJ, Lefkowitz EJ, King AMQ, Harrach B, Harrison RL, Knowles NJ, Kropinski AM, Krupovic M, Kuhn JH, Mushegian AR, Nibert M, Sabanadzovic S, Sanfacon H, Siddell SG, Simmonds P, Varsani A, Zerbini FM, Gorbalenya AE, Davison AJ (2017) Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2017). Arch Virol 162(8):2505–2538. doi:10.1007/s00705-017-3358-5

    Article  CAS  PubMed  Google Scholar 

  4. Kapoor A, Simmonds P, Cullen JM, Scheel TK, Medina JL, Giannitti F, Nishiuchi E, Brock KV, Burbelo PD, Rice CM, Lipkin WI (2013) Identification of a pegivirus (GB virus-like virus) that infects horses. J Virol 87(12):7185–7190. doi:10.1128/JVI.00324-13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Lyons S, Kapoor A, Schneider BS, Wolfe ND, Culshaw G, Corcoran B, Durham AE, Burden F, McGorum BC, Simmonds P (2014) Viraemic frequencies and seroprevalence of non-primate hepacivirus and equine pegiviruses in horses and other mammalian species. J Gen Virol 95(Pt 8):1701–1711. doi:10.1099/vir.0.065094-0

    Article  CAS  PubMed  Google Scholar 

  6. de Souza AJ, Malheiros AP, de Sousa ER, Moreira AC, Silva AL, das Chagas AA, Freitas PE, Gemaque BS, de Figueiredo HF, de Sa LR, dos Santos PD, Soares MC (2015) First report of equine Pegivirus in South America, Brazil. Acta Trop 152:56–59. doi:10.1016/j.actatropica.2015.08.014

    Article  PubMed  Google Scholar 

  7. Postel A, Cavalleri JM, Pfaender S, Walter S, Steinmann E, Fischer N, Feige K, Haas L, Becher P (2016) Frequent presence of hepaci and pegiviruses in commercial equine serum pools. Vet Microbiol 182:8–14. doi:10.1016/j.vetmic.2015.10.032

    Article  CAS  PubMed  Google Scholar 

  8. Lu G, Sun L, Xu T, He D, Wang Z, Ou S, Jia K, Yuan L, Li S (2016) First description of hepacivirus and pegivirus infection in domestic horses in China: a study in Guangdong Province, Heilongjiang Province and Hong Kong District. PLoS One 11(5):e0155662. doi:10.1371/journal.pone.0155662

    Article  PubMed  PubMed Central  Google Scholar 

  9. Ross-Thriepland D, Harris M (2015) Hepatitis C virus NS5A: enigmatic but still promiscuous 10 years on! J Gen Virol 96(Pt 4):727–738. doi:10.1099/jgv.0.000009

    Article  CAS  PubMed  Google Scholar 

  10. Simmonds P (2004) Genetic diversity and evolution of hepatitis C virus—15 years on. J Gen Virol 85(Pt 11):3173–3188. doi:10.1099/vir.0.80401-0

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Li Shoujun.

Ethics declarations

Funding

This work was supported by The National Natural Science Foundation of China (31402259), The Special Fund for Agro-scientific Research in the Public Interest (201303042), The National Key Research and Development Program of China (2016YFD0501004, 2016YFD0501010), The Promote Scientific and Technological Development Program (2013B040200032), The Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases (2013A061401013).

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

No studies involving human participants or animals performed by any authors are described in this article.

Additional information

Handling Editor: Zhenhai Chen.

The original version of this article was revised: The corresponding author name was incorrectly published in the original article and the same is corrected here.

A correction to this article is available online at https://doi.org/10.1007/s00705-017-3654-0.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 14 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lu, G., Fu, C., Huang, J. et al. Molecular characterization of a genetically divergent equine pegivirus strain identified in China. Arch Virol 163, 249–252 (2018). https://doi.org/10.1007/s00705-017-3602-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-017-3602-z

Navigation