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Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus

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Abstract

Alternaria fungi are important pathogens infecting a wide variety of organisms. Here, we report a novel double-stranded RNA (dsRNA) mycovirus named Alternaria botybirnavirus 1 (ABRV1) isolated from a phytopathogenic Alternaria sp. strain (SCFS-3) infecting a pear tree in China. ABRV1 has two dsRNA components (dsRNAs 1 and 2) with the sizes of 6,188 and 5,903 bp, containing two putative open reading frames encoding two polyproteins (202 and 192 kDa, respectively). The polyprotein encoded by ABRV1 dsRNA1 shares 41% amino acid (aa) sequence identity with the one encoded by dsRNA2 (instead of dsRNA1) of Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1). Conversely, the polyprotein encoded by ABRV1 dsRNA2 shares 46% aa sequence identity with the one (i.e., cap-pol fusion protein) encoded by SsBRV1 dsRNA1. ABRV1 has isometric spherical virus particles (~40 nm in diameter), putatively composed of the 60-, 70- and 80-kDa structural proteins. The genomic organization and phylogenetic analyses revealed that ABRV1 belongs to a newly proposed family “Botybirnaviridae”, and to our knowledge, this is the first report of a botybirnavirus infecting an Alternaria sp. strain.

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Acknowledgements

This work was financially supported by the earmarked fund for Pear Modern Agro-Industry Technology Research System (CARS-28-15) of the Chinese Ministry of Agriculture and the Fundamental Research Funds for the Central Universities (no. 2662016PY107).

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Author notes

  1. Jun Xiang and Min Fu contributed equally to the study.

Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Jun Xiang, Min Fu, Ni Hong & Guoping Wang

  2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Jun Xiang, Min Fu, Ni Hong, Lifeng Zhai, Feng Xiao & Guoping Wang

Authors
  1. Jun Xiang
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  2. Min Fu
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  3. Ni Hong
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  4. Lifeng Zhai
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  5. Feng Xiao
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  6. Guoping Wang
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Correspondence to Guoping Wang.

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The authors declare that they have no competing interests.

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No human or animal ethics approval was required for the completion of this study.

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Informed consent was obtained from all individual participants included in this study.

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Handling Editor: Robert H.A. Coutts.

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Xiang, J., Fu, M., Hong, N. et al. Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus. Arch Virol 162, 3907–3911 (2017). https://doi.org/10.1007/s00705-017-3543-6

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  • DOI: https://doi.org/10.1007/s00705-017-3543-6

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