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First complete genome sequence of a virulent bacteriophage infecting the opportunistic pathogen Serratia rubidaea

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Abstract

A Serratia rubidaea phage, vB_Sru IME250, was isolated from hospital sewage. The morphology suggested that phage vB_Sru IME250 should be classified as a member of the family Myoviridae. High-throughput sequencing revealed that the phage genome has 154,938 nucleotides and consists of 193 coding DNA sequences, 90 of which have putative functions. The genome of vB_Sru IME250 is a linear, double-stranded DNA with an average GC content of 40.04%. The phage has a relatively high similarity to Klebsiella phage 0507-KN2-1, with a genome coverage of 84%.

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Acknowledgements

The authors would like to thank Guangqian Pei, Yunfei Wang, Xiaodong Liu, Peng Shu, Shi Cheng and Wenli Liu for their help during this work. This research was supported by a Grant from the National Hi-Tech Research and Development (863) Program of China (Nos. 2012AA022003 and 2014AA021402), the China Mega-Project on Infectious Disease Prevention (Nos. 2013ZX10004605, 2011ZX10004001, 2013ZX10004607-004 and 2013ZX10004217-002-003), and the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113).

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Authors and Affiliations

  1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People’s Republic of China

    Shaozhen Xing, Xianglilan Zhang, Yong Huang, Zhiqiang Mi, Qiang Sun, Xiaoping An, Hang Fan & Yigang Tong

  2. Department of Immunology, Basic Medical College, Hebei Medical University, Shijiazhuang, 050017, People’s Republic of China

    Taping Ma & Lin Wei

  3. Key Laboratory of Immune Mechanism and Intervention on Serious Disease in Hebei Province, Shijiazhuang, 050017, People’s Republic of China

    Taping Ma & Lin Wei

  4. Department of Pathogenic Biology and Immunology, School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050000, China

    Shuhui Wu

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  1. Shaozhen Xing
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  8. Hang Fan
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  11. Yigang Tong
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Correspondence to Shuhui Wu, Lin Wei or Yigang Tong.

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Conflict of interest

All the authors have received research Grants from the National Hi-Tech Research and Development (863) Program of China (Nos. 2012AA022003 and 2014AA021402), the China Mega-Project on Infectious Disease Prevention (Nos. 2013ZX10004605, 2011ZX10004001, 2013ZX10004607-004 and 2013ZX10004217-002-003), and the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113). They declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This study was funded by the National Hi-Tech Research and Development (863) Program of China (Nos. 2012AA022003 and 2014AA021402), the China Mega-Project on Infectious Disease Prevention (Nos. 2013ZX10004605, 2011ZX10004001, 2013ZX10004607-004 and 2013ZX10004217-002-003), and the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113).

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Xing, S., Ma, T., Zhang, X. et al. First complete genome sequence of a virulent bacteriophage infecting the opportunistic pathogen Serratia rubidaea . Arch Virol 162, 2021–2028 (2017). https://doi.org/10.1007/s00705-017-3300-x

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  • DOI: https://doi.org/10.1007/s00705-017-3300-x

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