Abstract
The common bean is a host for a large number of begomoviruses (genus Begomovirus, family Geminiviridae) in the New World. Based on the current taxonomic criteria established for the genus Begomovirus, two new members of this genus infecting common bean (Phaseolus vulgaris) in Cuba are herein reported. The cloned bipartite genomes, composed of DNA-A and DNA-B, showed the typical organization of the New World begomoviruses. We propose the names common bean severe mosaic virus and common bean mottle virus for the new begomovirus species.
Similar content being viewed by others
References
Brown JK, Idris AM (2009) A new, virulent, broad host range bean-infecting begomovirus from Puerto Rico: Rhynchosia mild mosaic virus. Annu Rep Bean Improv Coop 52:30
Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JC, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619
Echemendía A, Ramos P, Peral R, Fuentes A, González G, Sanpedro J, Morales F (2001) Cuban isolate of bean golden yellow mosaic virus is a member of the Mesoamerican BGYMV Group. Plant Dis 85:1030
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797
Fiallo-Olivé E, Navas-Castillo J, Moriones E, Martínez-Zubiaur Y (2010) Two novel begomoviruses belonging to different lineages infecting Rhynchosia minima. Arch Virol 155:2053–2058
Fiallo-Olivé E, Márquez-Martín B, Hassan I, Chirinos DT, Geraud-Pouey F, Navas-Castillo J, Moriones E (2013) Complete genome sequences of two novel begomoviruses infecting common bean in Venezuela. Arch Virol 158:723–727
Fondong VN (2013) Geminivirus protein structure and function. Mol Plant Pathol 14:635–649
Fourment M, Gibbs AJ, Gibbs MJ (2008) SWeBLAST: a sliding window web-based BLAST tool for recombinant analysis. J Virol Methods 152:98–101
Idris A, Hiebert E, Bird J, Brown J (2003) Two newly described begomoviruses of Macroptilium lathyroides and common bean. Phytopathology 93:774–783
Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003
Martínez Zubiaur Y, Quiñones M, Fonseca D, Potter J, Maxwell D (2002) First report of tomato yellow leaf curl virus associated with beans, Phaseolus vulgaris, in Cuba. Plant Dis 86:814
Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9:e108277
Navas-Castillo J, Fiallo-Olivé E, Sánchez-Campos S (2011) Emerging virus diseases transmitted by whiteflies. Annu Rev Phytopathol 49:219–248
Permingeat HR, Romagnoli MV, Vallejos RH (1998) A simple method for isolating high yield and quality DNA from cotton (Gossypium hirsutum L.) leaves. Plant Mol Biol Rep 16:1–6
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Varsani A, Navas-Castillo J, Moriones E, Hernández-Zepeda C, Idris A, Brown JK, Zerbini FM, Martin DP (2014) Establishment of three new genera in the family Geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus. Arch Virol 159:2193–2203
Wyatt S, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293
Acknowledgements
L.C-S was supported by a MAEC-AECID fellowship from Ministerio de Asuntos Exteriores y de Cooperación, Spain. E.F-O. is recipient of a “Juan de la Cierva-Incorporación” contract (Ministerio de Economía y Competitividad, Spain).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflicts of interest.
Research involving human participants and/or animals
No part of this study was performed with human participants or animals by any of the authors.
Rights and permissions
About this article
Cite this article
Chang-Sidorchuk, L., González-Alvarez, H., Navas-Castillo, J. et al. Complete genome sequences of two novel bipartite begomoviruses infecting common bean in Cuba. Arch Virol 162, 1431–1433 (2017). https://doi.org/10.1007/s00705-016-3209-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-016-3209-9