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Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus

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Abstract

Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.

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Acknowledgments

We are grateful to Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran for financial support this study.

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Authors and Affiliations

  1. Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran

    Mohammad Amin Almasi & Galavizh Almasi

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  1. Mohammad Amin Almasi
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  2. Galavizh Almasi
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Correspondence to Mohammad Amin Almasi.

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The authors declare no conflicts of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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Almasi, M.A., Almasi, G. Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus. Arch Virol 162, 495–500 (2017). https://doi.org/10.1007/s00705-016-3116-0

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  • DOI: https://doi.org/10.1007/s00705-016-3116-0

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