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Reassortment among picobirnaviruses found in wolves

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Abstract

We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time that wolves are a potential reservoir for picobirnaviruses, which might play a role as enteric pathogens.

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Acknowledgments

We thank the ACHLI (Association for the Conservation of the Iberian Wolf Habitat) for funding wolf sampling, D. Castro (CTM/CIBIO) for genetic analysis of wolf scats, and D. Cadete, J. Bernardo and S. Pinto for conducting the scat survey. NCN was supported by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen). KCY was supported by the Interfaculty Council for Development Cooperation (IRO) from the KU Leuven. SR and RG were supported by ‘Fundacão para a Ciência e a Tecnologia (FCT, Portugal) (SFRH/BD/12291/2003 and IF/00564/2012, respectively).

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    Authors and Affiliations

    1. Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium

      Nádia Conceição-Neto, Mark Zeller, Claude Kwe Yinda, Elisabeth Heylen & Jelle Matthijnssens

    2. Laboratory for Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium

      Nádia Conceição-Neto, Claude Kwe Yinda & Marc Van Ranst

    3. Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal

      João Rodrigo Mesquita

    4. CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal

      João Rodrigo Mesquita, Francisco Álvares & Raquel Godinho

    5. cE3c, Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal

      Sara Roque & Francisco Petrucci-Fonseca

    6. Grupo Lobo, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal

      Sara Roque & Francisco Petrucci-Fonseca

    7. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal

      Raquel Godinho

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    1. Nádia Conceição-Neto
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    2. João Rodrigo Mesquita
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    3. Mark Zeller
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    4. Claude Kwe Yinda
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    5. Francisco Álvares
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    6. Sara Roque
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    7. Francisco Petrucci-Fonseca
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    8. Raquel Godinho
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    9. Elisabeth Heylen
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    10. Marc Van Ranst
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    11. Jelle Matthijnssens
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    Corresponding author

    Correspondence to Jelle Matthijnssens.

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    Conflict of interest

    The authors declare no conflict of interest.

    Ethical approval

    Wolf monitoring and sampling was approved by the “National Authority for Nature Conservation (ICNF)” in Portugal.

    Additional information

    N. Conceição-Neto and J. R. Mesquita contributed equally to this work.

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    Conceição-Neto, N., Mesquita, J.R., Zeller, M. et al. Reassortment among picobirnaviruses found in wolves. Arch Virol 161, 2859–2862 (2016). https://doi.org/10.1007/s00705-016-2987-4

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    • DOI: https://doi.org/10.1007/s00705-016-2987-4

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