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Great genetic diversity of rotaviruses detected in piglets with diarrhea in Thailand

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Abstract

A total of 491 fecal specimens collected from diarrheic piglets in Thailand from January 2011 to March 2014 were screened for group A rotavirus by RT-PCR assay. The G and P genotypes of the detected rotaviruses were determined by multiplex PCR or nucleotide sequencing. Group A rotaviruses were detected in 113 out of 491 (23.0 %) fecal specimens. A wide variety of G-P genotype combinations were identified, and G4P[13] was the most prevalent genotype combination (29.2 %), followed by G4P[23] (14.1 %), G5P[23] (11.5 %), G4P[6] (9.7 %), G3P[23] (7.0 %), G5P[13] (6.1 %), G3P[13] (4.4 %), G3P[6] (2.7 %), and G5P[6] (2.7 %). In addition, the other G-P combinations were also detected at a low percentage, including G3P[19], G4P[7], G9P[19], G9P[23], G9P[7], G4P[19], and G11P[13] strains. This study indicated that group A rotaviruses are a common causes of diarrhea in piglets and a great diversity of G and P genotype combinations are circulating in piglets in Thailand.

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Acknowledgements

This research was jointly supported by Grants from the Faculty of Medicine, Chiang Mai University (Medical Research Fund), Graduate School of Chiang Mai University, and Center of Excellence (Emerging and Re-emerging Diarrheal Viruses Research Center), Chiang Mai University, Chiang Mai Thailand. In addition, some parts of this project were supported by National Research Council of Thailand (NRCT).

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Arpaporn Yodmeeklin, Pattara Khamrin, Watchaporn Chuchaona, Wilaiporn Saikruang, Kattareeya Kumthip & Niwat Maneekarn

  2. Animal House Unit, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Aphisek Kongkaew

  3. Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand

    Ratchaya Vachirachewin

  4. Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan

    Shoko Okitsu & Hiroshi Ushijima

  5. Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Shoko Okitsu & Hiroshi Ushijima

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  1. Arpaporn Yodmeeklin
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Correspondence to Niwat Maneekarn.

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Yodmeeklin, A., Khamrin, P., Chuchaona, W. et al. Great genetic diversity of rotaviruses detected in piglets with diarrhea in Thailand. Arch Virol 161, 2843–2849 (2016). https://doi.org/10.1007/s00705-016-2976-7

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  • DOI: https://doi.org/10.1007/s00705-016-2976-7

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