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Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease

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Abstract

Metagenomic sequencing of three nasal swabs collected from 10- to 21-day-old pigs exhibiting unexplained acute respiratory disease from two different commercial production facilities in Oklahoma identified a novel genotype of porcine astrovirus 4 (PAstV-4). The genomes had only ~75 % nucleotide sequence identity to previously characterized PAstV-4 isolates, while the capsid-encoding ORF2 had only ~53 % amino acid sequence identity to reference strains. A TaqMan assay targeting the novel ORF2 gene found 21 % and 19 % incidence in nasal and fecal swabs, respectively, submitted for unrelated diagnostic testing. PAstV-4 RNA levels were significantly higher (P = 0.04) in nasal swabs, suggesting a possible atypical respiratory tropism.

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Acknowledgments

Funding was supported in part by the USDA’s Animal Health and Disease Research Program under the provisions of Section 1433 of Subtitle E, Title XIV of Public Law 95-113 and the Kansas State Veterinary Diagnostic Laboratory

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Authors and Affiliations

  1. Kansas State Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas, 66506, USA

    Aiswaria Padmanabhan & Ben M. Hause

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  1. Aiswaria Padmanabhan
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  2. Ben M. Hause
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Correspondence to Ben M. Hause.

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The authors report no conflict of interest.

Ethical approval

All animal samples used in this study were collected by veterinarians as part of their routine herd management practices and submitted to the Kansas State Veterinary Diagnostic Laboratory for diagnostic testing. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Padmanabhan, A., Hause, B.M. Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease. Arch Virol 161, 2575–2579 (2016). https://doi.org/10.1007/s00705-016-2937-1

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  • DOI: https://doi.org/10.1007/s00705-016-2937-1

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