Abstract
Bluetongue virus (BTV) has been found to trigger autophagy to favor its replication, but the underlying mechanisms have not been clarified. Here, we show that cellular energy metabolism is involved in BTV-induced autophagy. Cellular ATP synthesis was impaired by BTV1 infection, causing metabolic stress, which was responsible for activation of autophagy, since the conversion of LC3 and aggregation of GFP-LC3 (autophagy markers) were suppressed when infection-caused energy depletion was reversed via MP (metabolic substrate) treatment. The reduced virus yields with MP further supported this view. Overall, our findings suggest that BTV1-induced disruption of cellular energy metabolism contributes to autophagy, and this provides new insights into BTV-host interactions.
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Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (No. 31402207 and 31302065), Basic Scientific Research Foundation of Central Research Academies and Institutes (0302015008) and National High-Tech Research and Development Program of China (No. 2011AA10A212).
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Lv, S., Xu, Q., Sun, E. et al. Impaired cellular energy metabolism contributes to bluetongue-virus-induced autophagy. Arch Virol 161, 2807–2811 (2016). https://doi.org/10.1007/s00705-016-2924-6
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DOI: https://doi.org/10.1007/s00705-016-2924-6