Abstract
In this study, we describe the isolation and characterisation of the novel enterobacteria phage CAjan. This phage belongs to the order Caudovirales and the family Siphoviridae. The phage possesses a linear, double-stranded DNA genome consisting of 59,670 bp with a G+C content of 44.7 % and 91 predicted open reading frames (ORFs). Putative functions were assigned to 39 of the ORFs (37.4 %). The phage structural genes were furthermore functionally characterised by LC MS/MS. CAjan, together with Escherichia phage Seurat and Escherichia phage slur01, represent a novel and genetically distinct clade of Siphoviridae phages that could be considered to constitute a new phage genus. Despite limited sequence similarity, the phages in this group share a number of other common features, including genome structure and the presence of queuosine biosynthesis genes.
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This work was funded by Lundbeck grant R44-A4384 and a PHAGEMID project financed by the Danish Research Council for Technology and Production (4093-00198). All the authors declare no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.
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A. B. Carstens and W. Kot contributed equally to this work.
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Carstens, A.B., Kot, W., Lametsch, R. et al. Characterisation of a novel enterobacteria phage, CAjan, isolated from rat faeces. Arch Virol 161, 2219–2226 (2016). https://doi.org/10.1007/s00705-016-2901-0
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DOI: https://doi.org/10.1007/s00705-016-2901-0