Abstract
The complete nucleotide sequence of a new barley polerovirus, tentatively named barley virus G (BVG), which was isolated in Gimje, South Korea, has been determined using an RNA sequencing technique combined with polymerase chain reaction methods. The viral genomic RNA of BVG is 5,620 nucleotides long and contains six typical open reading frames commonly observed in other poleroviruses. Sequence comparisons revealed that BVG is most closely related to maize yellow dwarf virus-RMV, with the highest amino acid identities being less than 90 % for all of the corresponding proteins. These results suggested that BVG is a member of a new species in the genus Polerovirus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams MJ (1991) The distribution of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) in UK winter barley samples, 1987–1990. Plant Pathol 40:53–58
Baik BK, Ullrich SE (2008) Barley for food: characteristics, improvement, and renewed interest. J Cereal Sci 48:233–242
Domier LL (2012) Family—Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, 9th report of the ICTV. Elsevier/Academic Press, London, pp 1045–1053
Katis N, Tzavella-Klonari K, Adams MJ (1997) Occurrence of barley yellow mosaic and barley mild mosaic bymoviruses in Greece. Eur J Plant Pathol 103:281–284
Kreuze J (2014) siRNA Deep Sequencing and Assembly: Piecing Together Viral Infections. In: Gullino ML, Bonants PJM (eds) Detection and diagnostics of plant pathogens. Springer, Dordrecht, pp 21–38
Krueger EN, Beckett RJ, Gray SM, Miller WA (2013) The complete nucleotide sequence of the genome of Barley yellow dwarf virus-RMV reveals it to be a new Polerovirus distantly related to other yellow dwarf viruses. Front Microbiol 4:205. doi:10.3389/fmicb.2013.00205
Lee KJ, Kashiwazaki S, Hibi T, So IY (1996) Properties and capsid protein gene sequence of a Korean isolate of barley mild mosaic virus. Ann Phytopathol Soc Japan 62:397–401
Miller WA, Rasochová L (1997) Barley yellow dwarf viruses. Annu Rev Phytopathol 35:167–190
Nam M, Kim SM, Domier LL, Koh S, Moon JK, Choi HS, Kim HG, Moon JS, Lee SH (2009) Nucleotide sequence and genomic organization of a newly identified member of the genus Carmovirus, soybean yellow mottle mosaic virus, from soybean. Arch Virol 154:1679–1684
Pfeffer S, Dunoyer P, Heim F, Richards KE, Jonard G, Ziegler-Graff V (2002) P0 of beet western yellows virus is a suppressor of posttranscriptional gene silencing. J Virol 76:6815–6824
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Zhao F, Yoo RH, Lim S, Igori D, Lee SH, Moon JS (2015) Nucleotide sequence and genome organization of a new proposed crinivirus, tetterwort vein chlorosis virus. Arch Virol. doi:10.1007/s00705-015-2552-6
Acknowledgments
This work was supported by the National Institute of Crop Science (Project No. PJ01004204), Republic of Korea.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Funding
This study was funded by National Institute of Crop Science, Republic of Korea (Project No. PJ01004204).
Conflict of interest
All authors no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhao, F., Lim, S., Yoo, R.H. et al. The complete genomic sequence of a tentative new polerovirus identified in barley in South Korea. Arch Virol 161, 2047–2050 (2016). https://doi.org/10.1007/s00705-016-2881-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-016-2881-0