Abstract
Muscovy duck parvovirus (MDPV) infection is widespread in many Muscovy-duck-farming countries, leading to a huge economic loss. By means of overlapping peptides expressed in Escherichia coli in combination with Western blot, antigenic domains on the non-structural protein (NSP) of MDPV were identified for the first time. On the Western blot, the fragments NS(481-510), NS (501-530), NS (521-550), NS (541-570), NS (561-590), NS (581-610) and NS (601-627) were positive (the numbers in parentheses indicate the location of amino acids), and other fragments were negative. These seven fragments were also reactive in an indirect enzyme-linked immunosorbent assay (i-ELISA). We therefore conclude that a linear antigenic domain of the NSP is located at its C-terminal end (amino acid residues 481-627). These results may facilitate future investigations into the function of NSP of MDPV and the development of immunoassays for the diagnosis of MDPV infection.
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Acknowledgments
This study was supported by the Natural Science Foundation of Heilongjiang Province (Grant No. QC2014C034), Heilongjiang Province Ordinary Undergraduate Colleges and Universities Young Creative Talent Training Plan (Grant No. UNPYSCT-2015093) and Qiqihar University Young Teachers’ Scientific Research Support Program (Grant No. 2014k-Z02)
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Yu, Tf., Li, M., Yan, B. et al. Identification of antigenic domains in the non-structural protein of Muscovy duck parvovirus. Arch Virol 161, 2269–2272 (2016). https://doi.org/10.1007/s00705-016-2879-7
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DOI: https://doi.org/10.1007/s00705-016-2879-7