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Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus

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Abstract

The complete 6243-nucleotide sequence of a cucumber green mottle mosaic virus (CGMMV) isolate from bottle gourd in Zhejiang province, China, was determined. A full-length cDNA clone of this isolate was constructed by inserting the cDNA between the 35S promoter and the ribozyme in the binary plasmid pCB301-CH. A suspension of an Agrobacterium tumefaciens EHA105 clone carrying this construct was highly infectious in Nicotiana benthamiana and bottle gourd. Another infectious clone containing the green fluorescence protein (GFP) reporter gene was also successfully constructed. This study is the first report of the efficient use of agroinoculation for generating CGMMV infections.

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Acknowledgments

We thank Prof. Rongxiang Fang (Chinese Academy of Sciences) for providing the p35S-30B:GFP vector, and Dr. Xiaohan Mo for providing the pCB301-CH vector. This work was financially supported by the Special Fund for Agro-Scientific Research in the Public Interest (201303028, 201403032), the International Science & Technology Cooperation Program of China (2015DFA30700) and Fund for Research in the Public Interest of Zhejiang Province (2015C32042).

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Authors and Affiliations

  1. State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Hongying Zheng, Caili Xiao, Kelei Han, Jiejun Peng, Lin Lin, Yuwen Lu, Li Xie, Jianping Chen & Fei Yan

  2. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China

    Caili Xiao & Kelei Han

  3. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Xiaohua Wu, Pei Xu & Guojing Li

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  1. Hongying Zheng
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  2. Caili Xiao
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  3. Kelei Han
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  4. Jiejun Peng
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  5. Lin Lin
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  7. Li Xie
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  8. Xiaohua Wu
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  9. Pei Xu
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Corresponding authors

Correspondence to Jianping Chen or Fei Yan.

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Zheng, H., Xiao, C., Han, K. et al. Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus. Arch Virol 160, 2867–2872 (2015). https://doi.org/10.1007/s00705-015-2584-y

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  • DOI: https://doi.org/10.1007/s00705-015-2584-y

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