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Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain

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An Erratum to this article was published on 15 December 2016

Abstract

Tomato spotted wilt virus (TSWV) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method so far for TSWV disease control, but only two genes have been found to confer resistance against a wide spectrum of TSWV isolates: Sw-5 in tomato and Tsw in pepper. However, TSWV resistance-breaking isolates have emerged in different countries a few years after using resistant cultivars. In this paper, we report the first complete nucleotide sequences of three Spanish TSWV isolates with different biotypes according to their abilities to overcome resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 resistance breaking, SBR) and PVR (Tsw resistance-breaking, TBR). The genome of these TSWV isolates consisted of three segments: L (8913-8914 nt), M (4752-4825 nt) and (S 2924-2961 nt). Variations in nucleotide sequences and genomic RNA lengths among the different virus biotypes are reported here. Phylogenetic analysis of the five TSWV open reading frames showed evidence of reassortment between genomic segments of LL-N.05 and Pujol1TL3, which was supported by analysis with different recombination-detecting algorithms.

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Acknowledgments

D.E.D. was the recipient of a predoctoral FPU fellowship from the Spanish Ministry of Education, Culture and Sports. This work was funded in part by grants RTA2008-00010-C03 and RTA2013-00047-C02 from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and grants ACOMP/2009/103, ACOMP/2010/085 and ACOMP/2011/078 from Generalitat Valenciana. Work in the J.A.D. laboratory was supported by grant BIO2011-26741 from the Spanish Ministerio de Economía y Competitividad. We thank Verónica Aragonés (IBMCP, CSIC-UPV) for technical assistance.

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Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain

    Diana E. Debreczeni, Luis Galipienso & Luis Rubio

  2. Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de Valencia (COMAV-UPV), Camino de Vera s/n, 46022, Valencia, Spain

    Carmelo López & Salvador Soler

  3. Instituto de Biología Molecular y Celular de Plantas-Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, Valencia, Spain

    José Antonio Darós

  4. Euro-Mediterranean Institute of Science and Technology (IEMEST), Via E. Amari 123, 90139, Palermo, Italy

    Luis Galipienso & Luis Rubio

  5. Department of Plant Pathology, University of California, Davis, CA, 95616, USA

    Bryce W. Falk

Authors
  1. Diana E. Debreczeni
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  2. Carmelo López
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  3. José Aramburu
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  4. José Antonio Darós
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  5. Salvador Soler
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  6. Luis Galipienso
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  7. Bryce W. Falk
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  8. Luis Rubio
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Corresponding author

Correspondence to Luis Rubio.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s00705-016-3177-0.

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Debreczeni, D.E., López, C., Aramburu, J. et al. Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Arch Virol 160, 2117–2123 (2015). https://doi.org/10.1007/s00705-015-2453-8

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  • DOI: https://doi.org/10.1007/s00705-015-2453-8

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