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DRACO inhibits porcine reproductive and respiratory syndrome virus replication in vitro

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to cause substantial economic losses to the pig industry worldwide. Current vaccination strategies and antiviral drugs against PRRSV are still inadequate. Therefore, there is an urgent need for new antiviral strategies to control PRRSV. Double-stranded RNA (dsRNA) Activated Caspase Oligomerizer (DRACO) is a synthetic construct consisting of a dsRNA detection domain, an apoptosis induction domain, and a transduction tag. It has been shown to have broad-spectrum antiviral activity, but there have been no reports regarding its effect on PRRSV. Here, we demonstrate that DRACO exhibits robust antiviral activity against PRRSV infection by suppressing virus RNA and protein synthesis in both Marc-145 cells and porcine alveolar macrophages (PAMs). In addition, DRACO still exhibited strong anti-PRRSV activity when viral replication was enhanced by knockdown of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) in Marc-145 cells. Furthermore, in PAMs, DRACO was capable of inducing IL-6 expression and reducing Hsp70 expression, which might contribute to the inhibition of PRRSV infection. Collectively, our results imply that DRACO holds promise as a novel anti-PRRSV therapeutic drug.

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Acknowledgments

This work was supported by China Agriculture Research System (CASR-36) and China Spark Program (2011GA781001).

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    Authors and Affiliations

    1. State Key Laboratory of Biocontrol, Guangzhou Higher Education Mega Center, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou, 510006, Guangdong, People’s Republic of China

      Chunhe Guo, Luxi Chen, Delin Mo, Yaosheng Chen & Xiaohong Liu

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    1. Chunhe Guo
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    2. Luxi Chen
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    3. Delin Mo
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    4. Yaosheng Chen
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    5. Xiaohong Liu
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    Correspondence to Xiaohong Liu.

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    C. Guo and L. Chen contributed equally to this work.

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    Guo, C., Chen, L., Mo, D. et al. DRACO inhibits porcine reproductive and respiratory syndrome virus replication in vitro . Arch Virol 160, 1239–1247 (2015). https://doi.org/10.1007/s00705-015-2392-4

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