Skip to main content
SpringerLink
Log in

Complete genome sequence of two rabbit hemorrhagic disease virus variant b isolates detected on the Iberian Peninsula

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

We report the complete genome sequences of two isolates (RHDV-N11 and CBVal16) of variant rabbit hemorrhagic disease virus (RHDVb). Isolate N11 was detected in young domestic animals during a rabbit hemorrhagic disease (RHD) outbreak that occurred in 2011 on a rabbit farm in Navarra, Spain, while CBVal16 was isolated from a wild rabbit found dead in Valpaços, Northern Portugal, a year later. The viral sequences reported show 84.8–85.1 % and 78.3–78.5 % identity to RHDVAst/89 and RCV-A1 MIC-07, representative members of the pathogenic genogroup 1 RHDV and apathogenic rabbit calicivirus, respectively. In comparison with other RHDV isolates belonging to the previously known genogroups 1–6, RHDVb shows marked phenotypic differences, as it causes disease preferentially in young rabbits under 40 days of age and shows modified red blood cell agglutination profiles as well as antigenic differences that allow this variant to escape protection by the currently available vaccines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Abrantes J, van der Loo W, Le Pendu J, Esteves PJ (2012) Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review. Vet Res 43:12. doi:10.1186/1297-9716-43-12

    Article  PubMed Central  PubMed  Google Scholar 

  2. Alda F, Gaitero T, Suárez M, Merchán T, Rocha G, Doadrio I (2010) Evolutionary history and molecular epidemiology of rabbit haemorrhagic disease virus in the Iberian Peninsula and Western Europe. BMC Evol Biol 10(10):347. doi:10.1186/1471-2148-10-347

    Article  PubMed Central  PubMed  Google Scholar 

  3. Muller A, Freitas J, Silva E, Le Gall-Reculé G, Zwingelstein F, Abrantes J, Esteves PJ, Alves PC, van der Loo W, Kolodziejek J, Nowotny N, Thompson G (2009) Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula. Vet Microbiol 135(3–4):368–373. doi:10.1016/j.vetmic.2008.09.057

    Article  CAS  PubMed  Google Scholar 

  4. Dalton KP, Nicieza I, Abrantes J, Esteves PJ, Parra F (2014) Spread of new variant RHDV in domestic rabbits on the Iberian Peninsula. Vet Microbiol 169(1–2):67–73. doi:10.1016/j.vetmic.2013.12.015

    Article  PubMed  Google Scholar 

  5. Abrantes J, Lopes AM, Dalton KP, Parra F, Esteves PJ (2014) Detection of RHDVa on the Iberian Peninsula: isolation of an RHDVa strain from a Spanish rabbitry. Arch Virol 159(2):321–326. doi:10.1007/s00705-013-1808-2

    Article  CAS  PubMed  Google Scholar 

  6. Duarte MD, Henriques AM, Barros S, Luís T, Fagulha T, Ramos F, Fevereiro M (2014) New insight into the epidemiology of rabbit hemorrhagic disease viruses in Portugal: retrospective study reveals the circulation of genogroup 5 (G5) in Azores and discloses the circulation of G1 and G6 strains in mainland until 2008. Infect Genet Evol 27:149–155. doi:10.1016/j.meegid.2014.07.017

    Article  PubMed  Google Scholar 

  7. Le Gall-Reculé G, Zwingelstein F, Boucher S, Le Normand B, Plassiart G, Portejoie Y, Decors A, Bertagnoli S, Guérin JL, Marchandeau S (2011) Detection of a new variant of rabbit haemorrhagic disease virus in France. Vet Rec 168:137–138. doi:10.1136/vr.d697

    Article  PubMed  Google Scholar 

  8. Dalton KP, Nicieza I, Balseiro A, Muguerza MA, Rosell JM, Casais R, Álvarez ÁL, Parra F (2012) Variant rabbit hemorrhagic disease virus in young rabbits, Spain. Emerg Infect Dis 18:2009–2012

    Article  PubMed Central  PubMed  Google Scholar 

  9. Le Gall-Reculé G, Lavazza A, Marchandeau S, Bertagnoli S, Zwingelstein F, Cavadini P, Martinelli N, Lombardi G, Guérin JL, Lemaitre E, Decors A, Boucher S, Le Normand B, Capucci L (2013) Emergence of a new lagovirus related to rabbit haemorrhagic disease virus. Vet Res 8(44):81. doi:10.1186/1297-9716-44-81

    Article  Google Scholar 

  10. Abrantes J, Lopes AM, Dalton KP, Melo P, Correia JJ, Ramada M, Alves PC, Parra F, Esteves PJ (2013) New variant of rabbit hemorrhagic disease virus, Portugal, 2012–2013. Emerg Infect Dis 19(11):1900–1902. doi:10.3201/eid1911.130908

    Article  PubMed Central  PubMed  Google Scholar 

  11. Baily JL, Dagleish MP, Graham M, Maley M, Rocchi MS (2014) RHDV variant 2 presence detected in Scotland. Vet Rec 174:411. doi:10.1136/vr.g2781

    Article  PubMed  Google Scholar 

  12. Westcott DG, Frossard JP, Everest D, Dastjerdi A, Duff JP, Steinbach F, Choudhury B (2014) Incursion of RHDV2-like variant in Great Britain. Vet Rec 174(13):333. doi:10.1136/vr.g2345

    Article  PubMed  Google Scholar 

  13. Simpson V, Everest D, Westcott D (2014) RHDV variant 2 and Capillaria hepatica infection in rabbits. Vet Rec 174(19):486. doi:10.1136/vr.g3164

    Article  PubMed  Google Scholar 

  14. Rabbit Haemorrhagic Disease New variant of RHD virus now also detected in Germany Information from the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health. http://www.fli.bund.de/fileadmin/dam_uploads/Publikationen/FLI-Informationen/FLI-Information_RHD-Virus20131021-en.pdf

  15. Puggioni G, Cavadini P, Maestrale C, Scivoli R, Botti G, Ligios C, Le Gall-Reculé G, Lavazza A, Capucci L (2013) The new French 2010 rabbit hemorrhagic disease virus causes an RHD-like disease in the Sardinian Cape hare (Lepus capensis mediterraneus). Vet Res 7(44):96. doi:10.1186/1297-9716-44-96

    Article  Google Scholar 

  16. Ruvoen-Clouet N, Ganiere JP, Andre-Fontaine G, Blanchard D, Le Pendu J (2000) Binding of rabbit hemorrhagic disease virus to antigens of the ABH histo-blood group family. J Virol 74:11950–11954

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Nyström K, Le Gall-Reculé G, Grassi P, Abrantes J, Ruvoën-Clouet N, Le Moullac-Vaidye B, Lopes AM, Esteves PJ, Strive T, Marchandeau S, Dell A, Haslam SM, Le Pendu J (2011) Histo-blood group antigens act as attachment factors of rabbit hemorrhagic disease virus infection in a virus strain-dependent manner. PLoS Pathog 7:e1002188. doi:10.1371/journal.ppat.1002188

    Article  PubMed Central  PubMed  Google Scholar 

  18. Simmonds P, Karakasiliotis I, Bailey D, Chaudhry Y, Evans DJ, Goodfellow IG (2008) Bioinformaticand functional analysis of RNA secondary structure elements among different genera of human and animal caliciviruses. Nucleic Acids Res 36(8):2530–2546

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Morales M, Bárcena J, Ramírez MA, Boga JA, Parra F, Torres JM (2004) Synthesis in vitro of rabbit hemorrhagic disease virus subgenomic RNA by internal initiation on (–)sense genomic RNA: mapping of a subgenomic promoter. J Biol Chem 279(17):17013–17018

    Article  CAS  PubMed  Google Scholar 

  20. Joubert P, Pautigny C, Madelaine MF, Rasschaert D (2000) Identification of a new cleavage site of the 3C-like protease of rabbit haemorrhagic disease virus. J Gen Virol 81(Pt 2):481–488

    CAS  PubMed  Google Scholar 

  21. Meyers G, Wirblich C, Thiel HJ, Thumfart JO (2000) Rabbit hemorrhagic disease virus: genome organization and polyprotein processing of a calicivirus studied after transient expression of cDNA constructs. Virology 276(2):349–363

    Article  CAS  PubMed  Google Scholar 

  22. Bull R, White P (2010) Genome organization and recombination, Chapter 3. In: Hansman GS, Jiang XJ, Green KY (eds) Caliciviruses: molecular and cellular virology. Caister Academic Press, ISBN: 978-1-904455-63-9

  23. Sosnovtsev SV (2010) Proteolytic cleavage and viral proteins, Chapter 4. In: Hansman GS, Jiang XJ, Green KY (eds) Caliciviruses: molecular and cellular virology. Caister Academic Press, ISBN: 978-1-904455-63-9

  24. Sosnovtsev SV, Belliot G, Chang KO, Prikhodko VG, Thackray LB, Wobus CE, Karst SM, Virgin HW, Green KY (2006) Cleavage map and proteolytic processing of the murine norovirus nonstructural polyprotein in infected cells. J Virol 80(16):7816–7831

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Prof. Parra´s laboratory was supported by a grant (AGL2013-48550-C2-1-R) from the Spanish Ministerio de Economía y Competitividad, co-funded by the European Regional Development Fund (FEDER). Prof. Parra´s laboratory also acknowledges the sequencing services of the Servicios Científico Técnicos of the University of Oviedo. The Portuguese Foundation for Science and Technology (FCT) supported the doctoral fellowship of Ana M Lopes (ref.: SFRH/BD/78738/2011) and the FCT Investigator Grant of Joana Abrantes (ref.: IF/01396/2013). This work is funded by National Funds through FCT under the project (FCT-ANR/BIA-BIC/0043/2012. The project “Genomics Applied to Genetic Resources” co-financed by the North Portugal Regional Operation Programme 2007/2013 (ON.2-O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund, also supported this work.

Author information

Authors and Affiliations

  1. Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Oviedo, Spain

    K. P. Dalton, I. Nicieza, Á. L. Álvarez & F. Parra

  2. CIBIO-UP, Centro de Investigação em Biodiversidade e Recursos Genéticos-Universidade do Porto/InBIO, Laboratório Associado, Vairão, Portugal

    J. Abrantes, A. M. Lopes & P. J. Esteves

  3. Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal

    A. M. Lopes & P. J. Esteves

  4. INSERM, UMR892, CNRS, UMR6299, Université de Nantes, Nantes, France

    A. M. Lopes

  5. Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde (CESPU), Gandra, Portugal

    P. J. Esteves

Authors
  1. K. P. Dalton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Abrantes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Lopes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Nicieza
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Á. L. Álvarez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. J. Esteves
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Parra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. P. Dalton.

Additional information

Nucleotide sequence data reported are available in the GenBank database under the accession numbers KM878681 and KM979445.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dalton, K.P., Abrantes, J., Lopes, A.M. et al. Complete genome sequence of two rabbit hemorrhagic disease virus variant b isolates detected on the Iberian Peninsula. Arch Virol 160, 877–881 (2015). https://doi.org/10.1007/s00705-014-2329-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2329-3

Keywords

Search

Navigation