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Complete genome analysis of a novel recombinant isolate of potato virus Y from China

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Abstract

The complete sequence of GF_YL20, a potato virus Y (PVY) isolate from China, encodes a polyprotein of 3,061 amino acids. Sequence analysis indicates that GF_YL20 has a genomic structure different from previously reported PVY strains. It shares 99 % nucleotide sequence identity with PB209 (PVYN:O) except in VPg, but more than 97 % nucleotide sequence identity with the VPg of Mont (PVYN), PB312 (PVYNTN) and HN2 (SYR-I). Phylogenetic analysis indicates that GF_YL20 is a novel N:O recombinant with three recombination breakpoints.

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Acknowledgements

This work was supported by the China Agriculture Research System (Grant No. CARS-10-P11), the Natural Science Foundation of Fujian Province (Grant No. 2013J01088), the Educational Commission of Fujian Province (Grant No. JA12119), the program of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (Grant No. 2013IK299, 2013IK094), Fujian Natural Science Funds for Distinguished Young Scholar (Grant No. 2014J06008) and Fujian Entry-Exit Inspection and Quarantine Bureau (No. FK2011-07, FK2007-07), P. R. China. We thank Drs. Peter Thrall and Heinrich Josef Vetten for comments and suggestions about the manuscript.

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Authors and Affiliations

  1. Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China

    Fangluan Gao, Fei Chang, Fengyang Shi & Lianhui Xie

  2. Inspection and Quarantine Technology Center, Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou, 350001, P. R. China

    Jianguo Shen

  3. Key Lab for Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, P. R. China

    Jiasui Zhan

Authors
  1. Fangluan Gao
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  2. Fei Chang
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  3. Jianguo Shen
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  4. Fengyang Shi
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  5. Lianhui Xie
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  6. Jiasui Zhan
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Corresponding authors

Correspondence to Lianhui Xie or Jiasui Zhan.

Additional information

F. Gao and F. Chang contributed equally to this work.

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Fig. S1 Disease symptoms induced by GF_YL20 on potato (PDF 33 kb)

705_2014_2184_MOESM2_ESM.pdf

Fig. S2 Recombination analysis of the PVY GF_YL20 isolate by Simplot using PVYO (Oz), PVYN (Mont) and PVYN:O (PB209) as references. RJ1-RJ3 are the putative recombination breakpoints. (PDF 223 kb)

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Gao, F., Chang, F., Shen, J. et al. Complete genome analysis of a novel recombinant isolate of potato virus Y from China. Arch Virol 159, 3439–3442 (2014). https://doi.org/10.1007/s00705-014-2184-2

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  • DOI: https://doi.org/10.1007/s00705-014-2184-2

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