Abstract
The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5′ untranslated regions (UTR) of the two segments share regions of high sequence homology. Phylogenetic analysis indicates that this novel partitivirus, named Ustilaginoidea virens partitivirus 2 (UvPV2), can be assigned to the family Partitiviridae.
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This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest (200903039-3) from the Ministry of Agriculture of China.
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Supplementary Fig. S1 Phylograms of the UvPV2 CP. Multiple sequence alignments of the deduced amino acid sequences of UvPV2 and selected viruses belonging to families Chrysoviridae, Totiviridae and Partitiviridae were performed using MEGA 5.0. The phylogenic trees were analyzed using the NJ method with 1000 bootstrap replicates. The abbreviations of other virus names and GenBank accession numbers are as follows: Amasya cherry disease associated chrysovirus (ACDACV; YP-001531162); Aspergillus fumigatus chrysovirus (AfuCV; CAX48751); Botryotinia fuckeliana partitivirus 1 (BfPV1;YP-001109579); cherry chlorotic rusty spot associated chrysovirus (CCRSACV; AH03665); Coniothyrium minitans mycovirus (CmRV; YP-392466); Epichloe festucae virus 1 (EfV1; CAK02787); Heterobasidion RNA virus 8 (HetRV8; AFW17811); Helminthosporium victoriae 145S (HV145SV; YP-052859); Helicobasidium mompa totivirus 1-17 (HmT1-17; NC-005074); red clover cryptic virus 2 (RCCV-2; YP_007889824); Sphaeropsis sapinea RNA virus 2 (SsRV2; NP-047559); pepper cryptic virus 2 (PCV-2; AEJ07893); Primula malacoides virus China/Mar2007 (PmV1; EU195327).
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Jiang, Y., Luo, C., Jiang, D. et al. The complete genomic sequence of a second novel partitivirus infecting Ustilaginoidea virens . Arch Virol 159, 1865–1868 (2014). https://doi.org/10.1007/s00705-014-1991-9
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DOI: https://doi.org/10.1007/s00705-014-1991-9