Abstract
A novel double-stranded RNA (dsRNA) mycovirus, designated Verticillium dahliae partitivirus 1 (VdPV1), was isolated from a strain of the fungus Verticillium dahliae. The VdPV1 genome has two dsRNA genome segments. The larger segment (1768 bp) has a single open reading frame (ORF) with a conserved RNA-dependent RNA polymerase (RdRP) domain. The smaller segment (1587 bp) contains a single ORF encoding a putative coat protein. Analysis of its genomic structure indicated that VdPV1 is a new member of the genus Partitivirus. We report the full-length sequence of this partitivirus that infects Verticillium dahliae, the causal agent of verticillium wilt of cotton.
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Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Cao YF, Zhua XW, Xiang Y, Li DQ, Yang JR, Mao QZ, Chen JS (2011) Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb. Virus Res 159:73–78
Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA mycovirus from the white root rot fungus rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812
Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384
Heinze C (2012) A novel mycovirus from Clitocybe odora. Arch Virol 157:1831–1834
Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518
Klosterman SJ, Atallah ZK, Vallad GE, Subbarao KV (2009) Diversity, pathogenicity, and management of Verticillium species. Annu Rev Phytopathol 47:39–62
Kozlakidis Z, Herrero N, Ozkan S, Kanhayuwa L, Jamal A, Bhatti MF, Coutts RHA (2013) Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus. Arch Virol 158:263–266
Li XL, Li Y, Zhang TY (2003) Resistance screening and identification of cotton cultivars to Verticillium wilt disease. Plant Prot 29:18–20
Liu HQ, Fu YP, Xie JT, Cheng JS, Ghabrial SA, Li GQ, Peng YL, Yi XH, Jiang DH (2012) Evolutionary genomics of mycovirus-related dsRNA viruses reveals cross-family horizontal gene transfer and evolution of diverse viral lineages. BMC Evol Biol 12:91
Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128
Sasaki A, Kanematsu S, Onoue M, Oyama Y, Yoshida K (2006) Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8). Arch Virol 151:697–707
Xie J, Wei DM, Jiang DH, Fu YP, Li GQ, Ghabrial S, Peng YL (2006) Characterization of hypovirulence-associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum. J Gen Virol 87:241–249
Acknowledgment
This work was supported by National Science & Technology Pillar Program (2011BAD08A07) and the Central Public-Interest Scientific Institution Basal Research Fund, China (SJB1110). We are grateful to Jiatao Xie and Muhammad Ibrahim Khaskheli for their comments and critical reading of the manuscript.
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Feng, Z., Zhu, H., Li, Z. et al. Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb.. Arch Virol 158, 2621–2623 (2013). https://doi.org/10.1007/s00705-013-1774-8
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DOI: https://doi.org/10.1007/s00705-013-1774-8