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Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR

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Abstract

Potato virus M (PVM), pepino mosaic virus (PepMV), tomato mosaic virus (ToMV), and potato virus S (PVS) infect pepino and cause serious crop losses. In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.

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Acknowledgments

We thank Dr. M.A. Aranda and Dr. R.N. Sempere of Centro de Edafología y Biología Aplicada del Segura (CEBAS), Spanish National Research Council (CSIC), for their help with a PepMV-agroinfectious clone (PepXL6). This work was supported by Chinese Universities Scientific Fund (No. 2012YJ014) and the Special Fund for Agro-scientific Research in the Public Interest (No. 201203076).

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road No. 2, Haidian District, Beijing, 100193, People’s Republic of China

    Beibei Ge, Qian Li, Guojie Liu & Hongqing Wang

  2. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road No. 2, Haidian District, Beijing, 100193, People’s Republic of China

    Beibei Ge, Qian Li, Meiguang Lu & Shifang Li

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  1. Beibei Ge
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  2. Qian Li
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  3. Guojie Liu
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  4. Meiguang Lu
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Correspondence to Shifang Li or Hongqing Wang.

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Ge, B., Li, Q., Liu, G. et al. Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR. Arch Virol 158, 1181–1187 (2013). https://doi.org/10.1007/s00705-013-1604-z

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  • DOI: https://doi.org/10.1007/s00705-013-1604-z

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