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Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012

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Abstract

To analyze the epidemiology of PRRSV in Hubei Province of China, 668 serum samples collected from 14 pig-breeding farms were tested. We found that the PRRSV-positive rate was 5.24 % and that HP-PRRSV had become the dominant strain. To further investigate the genetic variation of PRRSV strains in this region, the complete gene sequences of nsp2, orf5, and orf7 from nine PRRSV strains collected during 2011-2012 were determined and compared with 33 known sequences. The results revealed that diverse HP-PRRSV strains are present in this region. An analysis of orf5 gene sequences showed that the strains collected during 2009-2010 formed a tightly clustered branch. When compared with the JXA1 strain, they had one mutation (V29 → A29) in a decoy epitope. Furthermore, we found that the number of potential N-glycosylation sites had apparently increased since 2006. These findings increase our knowledge of PRRSV epidemiology in Central China.

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Acknowledgments

This work was supported by Innovative Research Team in Hubei Center for Animal Disease Control and Prevention, the National Natural Science Foundation of China (Grants 30972161), and the Fundamental Research Funds for the Central Universities (Program No. 2011QC045).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Yuejun Shi, Zhenghui Xiong, Changqin Gu, Xueying Hu, Guofu Cheng & Wanpo Zhang

  2. Hubei Center for Animal Disease Control and Prevention, Wuhan, 430070, Hubei, China

    Zhibin Hu, Yanjun Zhou, Xin Jin & Nianhua Song

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  1. Yuejun Shi
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  2. Zhibin Hu
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  3. Zhenghui Xiong
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  4. Yanjun Zhou
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  5. Xin Jin
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  6. Changqin Gu
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  7. Xueying Hu
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  8. Guofu Cheng
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  9. Nianhua Song
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  10. Wanpo Zhang
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Correspondence to Wanpo Zhang.

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Shi, Y., Hu, Z., Xiong, Z. et al. Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012. Arch Virol 158, 717–721 (2013). https://doi.org/10.1007/s00705-012-1542-1

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  • DOI: https://doi.org/10.1007/s00705-012-1542-1

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