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Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China

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Abstract

A previous investigation showed that MX10 virus, recently isolated in China, belongs to the Oriental-Australian (O/A) genotype of Sindbis virus (SINV) (Wang Jinglin, 2011, ATMH). Similar to the MRE16 isolate, the prototype O/A genotype of SINV, two derivate viruses with obviously different plaque morphologies were derived from MX10 virus, which were accordingly denoted as MX10-LP and MX10-SP. MX10-LP virus exhibited higher neurovirulence in neonatal mice than MX10-SP virus. Analysis of the complete genome revealed seven nucleotide differences between MX10-LP and MX10-SP. Compared with MRE16 virus, MRE16SP virus has a deletion of 30 aa in the E2 gene (200-229), which has been shown to be the molecular basis for the different plaque morphology. However, the MX10-SP virus did not have the 30-amino-acid deletion in the E2 gene. These results demonstrate that the molecular basis for the different plaque morphology of MX10 virus, the first strain of the O/A genotype of SINV isolated from China, is different from that of the prototype MRE16 virus.

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Acknowledgments

This work was supported by the grants from the National Natural Science Foundation of China (No. 81160353; 30970160), China Mega-Project for Infectious Disease (2011ZX10004-001), a Development Grant of State Key Laboratory for Infectious Disease Prevention and Control (2008SKLID105; 2011SKLID205) and China CDC-US CDC Cooperative Agreement U19-GH000004.

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Authors and Affiliations

  1. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Viral Disease Control and Prevention, Beijing, 100052, People’s Republic of China

    Wang Jinglin, Zhu Wuyang, Shihong Fu, Li Wenjuan & Guodong Liang

  2. Yunnan Institute of Endemic Disease Control and Prevention, Dali, 671000, Yunnan, People’s Republic of China

    Wang Jinglin & Zhang Hailin

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  1. Wang Jinglin
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  2. Zhu Wuyang
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  3. Shihong Fu
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  4. Li Wenjuan
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  5. Zhang Hailin
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  6. Guodong Liang
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Corresponding author

Correspondence to Guodong Liang.

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W. Jinglin and Z. Wuyang contributed equally to this work.

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Jinglin, W., Wuyang, Z., Fu, S. et al. Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China. Arch Virol 158, 71–75 (2013). https://doi.org/10.1007/s00705-012-1464-y

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  • DOI: https://doi.org/10.1007/s00705-012-1464-y

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