Abstract
Faecal samples obtained from either asymptomatic or diarrhoeic calves in Italy were screened for bovine kobuviruses (BKVs) using specific primers. BKV RNA was detected in 4.9 % of the samples, with higher positivity rates in diarrhoeic calves (5.3 %) than in asymptomatic animals (4.8 %), although the difference was not statistically significant. Upon sequence analysis, all of the Italian viruses formed a tight group along with BKV-like sequences previously detected in Thailand and Japan.
References
Barry AF, Ribeiro J, Alfieri AF, van der Poel WH, Alfieri AA (2011) First detection of kobuvirus in farm animals in Brazil and the Netherlands. Infect Genet Evol 11:1811–1814
Greninger AL, Runckel C, Chiu CY, Haggerty T, Parsonnet J, Ganem D, DeRisi JL (2009) The complete genome of klassevirus—a novel picornavirus in pediatric stool. Virol J 6:82
Holtz LR, Finkbeiner SR, Zhao G, Kirkwood CD, Girones R, Pipas JM, Wang D (2009) Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread. Virol J 6:86
Jeoung HY, Lim JA, Jeong W, Oem JK, An DJ (2011) Three clusters of bovine kobuvirus isolated in Korea, 2008–2010. Virus Genes 42:402–406
Kapoor A, Simmonds P, Dubovi EJ, Qaisar N, Henriquez JA, Medina J, Shields S, Lipkin WI (2011) Characterization of a canine homolog of human Aichi virus. J Virol 85:11520–11525
Khamrin P, Maneekarn N, Kongkaew A, Kongkaew S, Okitsu S, Ushijima H (2009) Porcine kobuvirus in piglets, Thailand. Emerg Infect Dis 15:2075–2076
Khamrin P, Maneekarn N, Peerakome S, Okitsu S, Mizuguchi M, Ushijima H (2008) Bovine kobuviruses from cattle with diarrhea. Emerg Infect Dis 14:985–986
King AMQ, Brown F, Christian P, et al. (1999) Picornaviridae. In: van Regenmortel MHV, Faquet CM, Bishop DHL, Carsten EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy: seventh report of the international committee on taxonomy of viruses. Academic Press Inc., New York, p 996
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:153–163
Lee MH, Jeoung HY, Lim JA, Song JY, Song DS, An DJ (2012) Kobuvirus in South Korean black goats. Virus Genes. doi:10.1007/s11262-012-0745-6
Mauroy A, Scipioni A, Mathijs E, Thys C, Thiry E (2009) Molecular detection of kobuviruses and recombinant noroviruses in cattle in continental Europe. Arch Virol 154:1841–1845
Oh DY, Silva PA, Hauroeder B, Diedrich S, Cardoso DD, Schreier E (2006) Molecular characterization of the first Aichi viruses isolated in Europe and in South America. Arch Virol 151:1199–1206
Park SJ, Kim HK, Song DS, Moon HJ, Park BK (2011) Molecular detection and genetic characterization of kobuviruses in fecal samples collected from diarrheic cattle in Korea. Infect Genet Evol 11:1178–1182
Pham NT, Khamrin P, Nguyen TA, Kanti DS, Phan TG, Okitsu S, Ushijima H (2007) Isolation and molecular characterization of Aichi viruses from fecal specimens collected in Japan, Bangladesh, Thailand, and Vietnam. J Clin Microbiol 45:2287–2288
Phan TG, Kapusinszky B, Wang C, Rose RK, Lipton HL, Delwart EL (2011) The fecal viral flora of wild rodents. PLoS Pathog 7:e1002218. doi:10.1371/journal.ppat.1002218
Reuter G, Boldizsár A, Kiss I, Pankovics P (2008) Candidate new species of kobuvirus in porcine hosts. Emerg Infect Dis 14:1968–1970
Reuter G, Boldizsár Á, Pankovics P (2009) Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in genus Kobuvirus, family Picornaviridae. Arch Virol 154:101–108
Reuter G, Boros A, Pankovics P (2011) Kobuviruses—a comprehensive review. Rev Med Virol 21:32–41
Reuter G, Boros A, Pankovics P, Egyed L (2010) Kobuvirus in domestic sheep, Hungary. Emerg Infect Dis 16:869–870
Reuter G, Egyed L (2009) Bovine kobuvirus in Europe. Emerg Infect Dis 15:822–823
Sdiri-Loulizi K, Hassine M, Gharbi-Khelifi H, Sakly N, Chouchane S, Guediche MN, Pothier P, Aouni M, Ambert-Balay K (2009) Detection and genomic characterization of Aichi viruses in stool samples from children in Monastir, Tunisia. J Clin Microbiol 47:2275–2278
Yamashita T, Ito M, Kabashima Y, Tsuzuki H, Fujiura A, Sakae K (2003) Isolation and characterization of a new species of kobuvirus associated with cattle. J Gen Virol 84:3069–3077
Yamashita T, Kobayashi S, Sakae K, Nakata S, Chiba S, Ishihara Y, Isomura S (1991) Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis. J Infect Dis 164:954–957
Yamashita T, Sakae K, Tsuzuki H, Suzuki Y, Ishikawa N, Takeda N, Miyamura T, Yamazaki S (1998) Complete nucleotide sequence and genetic organization of Aichi virus, a distinct member of the Picornaviridae associated with acute gastroenteritis in humans. J Virol 72:8408–8841
Yu JM, Jin M, Zhang Q, Li HY, Li DD, Xu ZQ, Li JS, Cui SX, Yang SH, Liu N, Duan ZJ (2009) Candidate porcine kobuvirus, China. Emerg Infect Dis 15:823–825
Acknowledgments
We thank Dr. Giovanni Befacchia for the collection of faecal specimens. The work was financed by grants from the University of Teramo, Italy, and from the Italian Ministry of University and Research.
Conflict of interest
All authors declare that there are no financial or other relationships that might lead to a conflict of interest. All authors have seen and approved the manuscript and have contributed significantly to the work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Di Martino, B., Di Profio, F., Di Felice, E. et al. Molecular detection of bovine kobuviruses in Italy. Arch Virol 157, 2393–2396 (2012). https://doi.org/10.1007/s00705-012-1439-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-012-1439-z