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Differential expression of the Toll-like receptor pathway and related genes of chicken bursa after experimental infection with infectious bursa disease virus

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Abstract

Infectious bursa disease virus causes an acute infection in bursal B cells. The Toll-like receptor (TLR) signaling pathway plays a key role in innate immunity during virus infection. In this study, an Agilent microarray was used to investigate different transcriptional profiles of the TLR pathway and related genes of chicken bursa at 48 h after infection with IBDV, compared with simulated infection. Expression of >58 genes changed significantly. Forty-six genes associated with chicken bursa proinflammatory effects, chemotactic effects, and T-cell stimulation were upregulated, which meant enhancement of these features. Twelve genes that are related to proliferation and differentiation of bursal cells were downregulated, implying suppression of these features. These results revealed that genes of the TLR pathway play an important role in the pathogenicity of IBDV infection. The findings are helpful for understanding the molecular basis of viral pathogenesis and the underlying mechanism of the host antiviral response.

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Acknowledgments

This work was supported by grants from National Twelve-Five Technological Supported Plan of China (No. 2011BAD34B01) and public service sectors agriculture research projects (No. 201003060-9/10). We are thankful for the help from the members of China Agricultural University & Beijing University of Agriculture Traditional Chinese Veterinary Medicine (CAU-BUA TCVM) teaching and research team. This study was approved by the Beijing Administration Office of Laboratory Animals, and all of our experiments were operated at Veterinary Laboratory Biosafety Level 2. Special thanks to Advanced Throughput Inc. for their assistance with the chip experiments and data analysis.

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None of the authors has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of this paper.

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  1. College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China

    Xinfeng Guo, Liqiong Wang, Defeng Cui, Wenke Ruan, Fenghua Liu & Huanrong Li

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  1. Xinfeng Guo
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Correspondence to Fenghua Liu or Huanrong Li.

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X. Guo and L. Wang contributed equally to this work.

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Guo, X., Wang, L., Cui, D. et al. Differential expression of the Toll-like receptor pathway and related genes of chicken bursa after experimental infection with infectious bursa disease virus. Arch Virol 157, 2189–2199 (2012). https://doi.org/10.1007/s00705-012-1403-y

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