Skip to main content
SpringerLink
Log in

Genetic characterization of a new subtype of Hantaan virus isolated from a hemorrhagic fever with renal syndrome (HFRS) epidemic area in Hubei Province, China

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

To characterize hantaviruses currently circulating in the hemorrhagic fever with renal syndrome (HFRS) epidemic area of Hubei Province, rodents were captured and serum samples were collected from several HFRS patients. The partial S segment of the hantaviruses amplified from two serum samples had a high degree of sequence identity to the corresponding hantavirus strain isolated from Apodemus agrarius (designated as HV004). The complete S, M, and L segment sequences of HV004 were determined. The sequence identities between strain HV004 and other Hantaan viruses (HTNVs) were 83 %–90 % at the nucleotide level and 95 %–99 % at the amino acid level. Phylogenetic analysis showed that HV004 belonged to a new HTNV lineage. These data suggest the presence of a new HTNV subtype, which probably caused the HFRS cases in the endemic area of Hubei Province.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Plyusnin A, Vapalahti O, Vaheri A (1996) Hantaviruses: genome structure, expression and evolution. J Gen Virol 77(Pt 11):2677–2687

    Article  PubMed  CAS  Google Scholar 

  2. Easterbrook JD, Klein SL (2008) Immunological mechanisms mediating hantavirus persistence in rodent reservoirs. PLoS Pathog 4(11):e1000172

    Article  PubMed  Google Scholar 

  3. Kang HJ, Bennett SN, Hope AG, Cook JA, Yanagihara R (2011) Shared ancestry between a newfound mole-borne hantavirus and hantaviruses harbored by cricetid rodents. J Virol 85(15):7496–7503

    Article  PubMed  CAS  Google Scholar 

  4. Kang HJ, Kadjo B, Dubey S, Jacquet F, Yanagihara R (2011) Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Cote d’Ivoire. Virol J 8:373

    Article  PubMed  Google Scholar 

  5. Weiss S, Witkowski PT, Auste B, Nowak K, Weber N, Fahr J, Mombouli JV, Wolfe ND, Drexler JF, Drosten C, Klempa B, Leendertz FH, Kruger DH (2012) Hantavirus in bat Sierra Leone. Emerg Infect Dis 18(1):159–161

    Article  PubMed  Google Scholar 

  6. Plyusnin A, Morzunov SP (2001) Virus evolution and genetic diversity of hantaviruses and their rodent hosts. Curr Top Microbiol Immunol 256:47–75

    Article  PubMed  CAS  Google Scholar 

  7. Song G (1999) Epidemiological progresses of hemorrhagic fever with renal syndrome in China. Chin Med J 112(5):472–477

    PubMed  CAS  Google Scholar 

  8. Zhang YZ, Xiao DL, Wang Y, Wang HX, Sun L, Tao XX, Qu YG (2004) The epidemic characteristics and preventive measures of hemorrhagic fever with syndromes in China. Zhonghua liu xing bing xue za zhi 25(6):466–469 (in Chinese)

    PubMed  Google Scholar 

  9. Zhang YZ, Zou Y, Fu ZF, Plyusnin A (2010) Hantavirus infections in humans and animals China. Emerg Infect Dis 16(8):1195–1203

    Article  PubMed  Google Scholar 

  10. Guo WP, Lin XD, Wang W, Zhang XH, Chen Y, Cao JH, Ni QX, Li WC, Li MH, Plyusnin A, Zhang YZ (2011) A new subtype of Thottapalayam virus carried by the Asian house shrew (Suncus murinus) in China. Infect Genet Evol 11(8):1862–1867

    Article  PubMed  CAS  Google Scholar 

  11. Zhang YZ, Zou Y, Yan YZ, Hu GW, Yao LS, Du ZS, Jin LZ, Liu YY, Li MH, Chen HX, Fu ZF (2007) Detection of phylogenetically distinct Puumala-like viruses from red-grey vole Clethrionomys rufocanus in China. J Med Virol 79(8):1208–1218

    Article  PubMed  CAS  Google Scholar 

  12. Zou Y, Wang JB, Gaowa HS, Yao LS, Hu GW, Li MH, Chen HX, Plyusnin A, Shao R, Zhang YZ (2008) Isolation and genetic characterization of hantaviruses carried by Microtus voles in China. J Med Virol 80(4):680–688

    Article  PubMed  CAS  Google Scholar 

  13. Li Q, Yang ZQ, Qu H, Xiao H (2005) Variability of G1 gene of hantaviruses occurring in the Hubei Province, P.R. China from 1985 to 2000. Acta virologica 49(1):51–57

    PubMed  CAS  Google Scholar 

  14. Chen XX, Yang ZQ, Liu JJ (2001) Genetic Variation of G1 Glycoprotein- Encoding Region of Hantaan Virus Hubei Strain. Wuhan Univ J 22:289–292 (in Chinese)

    CAS  Google Scholar 

  15. Zhao J, Lin F (2002) Analysis on the Surveillance result of hemorrhagic fever with renal syndrome in 2001 from Hubei province. Dis Surveill 10(4):369–372 (in Chinese)

    Google Scholar 

  16. Li Q, Luo F, Yang ZQ (2008) Humoral cross-reactivity of hantaan viruses to the nucleocapsid protein of Puumala viruses and Dobrava viruses in Hubei province. Immunol J 24(4):438–441 (in Chinese)

    CAS  Google Scholar 

  17. Xiong HR, Li Q, Chen W, Liu DY, Ling JX, Liu J, Liu YJ, Zhang Y, Yang ZQ (2011) Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses. Eur J Clin Microbiol Infect Dis 30(5):645–651

    Article  PubMed  Google Scholar 

  18. Deng HY, Luo F, Shi LQ, Zhong Q, Liu YJ, Yang ZQ (2009) Efficacy of arbidol on lethal hantaan virus infections in suckling mice and in vitro. Acta pharmacol Sin 30(7):1015–1024

    Article  PubMed  CAS  Google Scholar 

  19. Bharadwaj M, Nofchissey R, Goade D, Koster F, Hjelle B (2000) Humoral immune responses in the hantavirus cardiopulmonary syndrome. J Infect Dis 182(1):43–48

    Article  PubMed  CAS  Google Scholar 

  20. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947–2948

    Article  PubMed  CAS  Google Scholar 

  21. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Series 41:95–98

    CAS  Google Scholar 

  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  PubMed  CAS  Google Scholar 

  23. Bohlman MC, Morzunov SP, Meissner J, Taylor MB, Ishibashi K, Rowe J, Levis S, Enria D, St Jeor SC (2002) Analysis of hantavirus genetic diversity in Argentina: S segment-derived phylogeny. J Virol 76(8):3765–3773

    Article  PubMed  CAS  Google Scholar 

  24. Schmaljohn CS (1996) Molecular biology of hantaviruses. In: Elliott RM (ed) The Bunyaviridae. Plenum Press, New York, pp 63–90

    Google Scholar 

  25. Zou Y, Hu J, Wang ZX, Wang DM, Li MH, Ren GD, Duan ZX, Fu ZF, Plyusnin A, Zhang YZ (2008) Molecular diversity and phylogeny of Hantaan virus in Guizhou, China: evidence for Guizhou as a radiation center of the present Hantaan virus. J Gen Virol 89(Pt 8):1987–1997

    Article  PubMed  CAS  Google Scholar 

  26. Plyusnin A, Beaty BJ, Elliott RM, Goldbach R, Kormelink R, Lundkvist Å, Schmaljohn CS, Tesh RB (2011) Bunyaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego, pp 693–709

    Google Scholar 

  27. Elgh F, Lundkvist A, Alexeyev OA, Wadell G, Juto P (1996) A major antigenic domain for the human humoral response to Puumala virus nucleocapsid protein is located at the amino-terminus. J Virol Methods 59(1–2):161–172

    Article  PubMed  CAS  Google Scholar 

  28. Lundkvist A, Meisel H, Koletzki D, Lankinen H, Cifire F, Geldmacher A, Sibold C, Gott P, Vaheri A, Kruger DH, Ulrich R (2002) Mapping of B-cell epitopes in the nucleocapsid protein of Puumala hantavirus. Viral Immunol 15(1):177–192

    Article  PubMed  CAS  Google Scholar 

  29. Ogino M, Yoshimatsu K, Ebihara H, Araki K, Lee BH, Okumura M, Arikawa J (2004) Cell fusion activities of Hantaan virus envelope glycoproteins. J Virol 78(19):10776–10782

    Article  PubMed  CAS  Google Scholar 

  30. Shi X, Elliott RM (2004) Analysis of N-linked glycosylation of hantaan virus glycoproteins and the role of oligosaccharide side chains in protein folding and intracellular trafficking. J Virol 78(10):5414–5422

    Article  PubMed  CAS  Google Scholar 

  31. Zheng F, Ma L, Shao L, Wang G, Chen F, Zhang Y, Yang S (2007) Defining the N-linked glycosylation site of Hantaan virus envelope glycoproteins essential for cell fusion. J Microbiol 45(1):41–47

    PubMed  CAS  Google Scholar 

  32. Lundkvist A, Niklasson B (1992) Bank vole monoclonal antibodies against Puumala virus envelope glycoproteins: identification of epitopes involved in neutralization. Arch Virol 126(1–4):93–105

    Article  PubMed  CAS  Google Scholar 

  33. Lundkvist A, Hukic M, Horling J, Gilljam M, Nichol S, Niklasson B (1997) Puumala and Dobrava viruses cause hemorrhagic fever with renal syndrome in Bosnia-Herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera. J Med Virol 53(1):51–59

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National High Technology Research and Development 863 Program of China (No. 2007AA02Z465), a grant from the National Natural Science Foundation of China (NSFC project No. 30770096) and a grant from Natural Science Foundation of Hubei Province (2011CDB467).

Author information

Authors and Affiliations

  1. State Key Laboratory of Virology, Institute of Medical Virology, School of Medicine Wuhan University, 185 Donghu Road, Wuhan, 430071, People’s Republic of China

    Jin-lin Li, Jia-xin Ling, Dong-ying Liu, Jing Liu, Yuan-yuan Liu, Fei Wei, Fan Luo, Wen Chen, Yi-hui Zhang, Hai-rong Xiong, Wei Hou & Zhan-qiu Yang

  2. Department of Microbiology, School of Medicine, Wuhan University, Wuhan, 430071, Hubei, People’s Republic of China

    Dong-ying Liu

Authors
  1. Jin-lin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jia-xin Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dong-ying Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuan-yuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fei Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fan Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wen Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yi-hui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hai-rong Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Wei Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Zhan-qiu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhan-qiu Yang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 2157 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, Jl., Ling, Jx., Liu, Dy. et al. Genetic characterization of a new subtype of Hantaan virus isolated from a hemorrhagic fever with renal syndrome (HFRS) epidemic area in Hubei Province, China. Arch Virol 157, 1981–1987 (2012). https://doi.org/10.1007/s00705-012-1382-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-012-1382-z

Keywords

Search

Navigation