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Isolation and characterization of a Chinese strain of human enterovirus 74 from a healthy child in the Tibet Autonomous Region of China

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Abstract

Human enterovirus 74 (HEV74) is a recently described serotype within the species Human enterovirus B (HEV-B). Few nucleotide sequences of HEV74 are available, and only one complete genome sequence (the prototype strain) has been published. In this study, we report the complete genome sequence of an HEV74 strain isolated from a healthy child during a stool survey in the Tibet Autonomous Region of China. The results indicated that HEV74 may be a prevalent and common enterovirus type, and that HEV74 is globally distributed, especially in Asia. Sequence analysis revealed high variability among HEV74 strains and indicated frequent recombination within HEV-B.

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References

  1. Bailly JL, Cardoso MC, Labbé A, Peigue-Lafeuille H (2004) Isolation and identification of an enterovirus 77 recovered from a refugee child from Kosovo, and characterization of the complete virus genome. Virus Res 99:147–155

    Article  PubMed  CAS  Google Scholar 

  2. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98

    CAS  Google Scholar 

  3. Harvala H, Sharp CP, Ngole EM, Delaporte E, Peeters M, Simmonds P (2011) Detection and genetic characterization of enteroviruses circulating among wild populations of chimpanzees in Cameroon: relationship with human and simian enteroviruses. J Virol 85:4480–4486

    Article  PubMed  CAS  Google Scholar 

  4. Junttila N, Lévêque N, Kabue JP, Cartet G, Mushiya F, Muyembe-Tamfum JJ, Trompette A, Lina B, Magnius LO, Chomel JJ, Norder H (2007) New enteroviruses, EV-93 and EV-94, associated with acute flaccid paralysis in the Democratic Republic of the Congo. J Med Virol 79:393–400

    Article  PubMed  Google Scholar 

  5. Knowles NJ, Hovi T, Hyypiä T, King AMQ, Lindberg M, Pallansch MA, Palmenberg AC, Simmonds P, Skern T, Stanway G, Yamashita T, Zell R (2011) Picornaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, Diego, pp 855–880

    Google Scholar 

  6. Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  PubMed  CAS  Google Scholar 

  7. Norder H, Bjerregaard L, Magnius LO (2002) Open reading frame sequence of an Asian enterovirus 73 strain reveals that the prototype from California is recombinant. J Gen Virol 83:1721–1728

    PubMed  CAS  Google Scholar 

  8. Norder H, Bjerregaard L, Magnius L, Lina B, Aymard M, Chomel JJ (2003) Sequencing of ‘untypable’ enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types. J Gen Virol 84:827–836

    Article  PubMed  CAS  Google Scholar 

  9. Oberste MS, Maher K, Kilpatrick DR, Pallansch MA (1999) Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73:1941–1948

    PubMed  CAS  Google Scholar 

  10. Oberste MS, Maher K, Flemister MR, Marchetti G, Kilpatrick DR, Pallansch MA (2000) Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol 38:1170–1174

    PubMed  CAS  Google Scholar 

  11. Oberste M, Schnurr D, Maher K, al-Busaidy S, Pallansch M (2001) Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype. J Gen Virol 82:409–416

    PubMed  CAS  Google Scholar 

  12. Oberste MS, Nix WA, Maher K, Pallansch MA (2003) Improved molecular identification of enteroviruses by RT-PCR and amplicon sequencing. J Clin Virol 26:375–377

    Article  PubMed  CAS  Google Scholar 

  13. Oberste MS, Michele SM, Maher K, Schnurr D, Cisterna D, Junttila N, Uddin M, Chomel JJ, Lau CS, Ridha W, al-Busaidy S, Norder H, Magnius LO, Pallansch MA (2004) Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75. J Gen Virol 85:3205–3212

    Article  PubMed  CAS  Google Scholar 

  14. Oberste MS, Maher K, Nix WA, Michele SM, Uddin M, Schnurr D, al-Busaidy S, Akoua-Koffi C, Pallansch MA (2007) Molecular identification of 13 new enterovirus types, EV79–88, EV97, and EV100–101, members of the species Human Enterovirus B. Virus Res 128:34–42

    Article  PubMed  CAS  Google Scholar 

  15. Oberste MS, Maher K, Patterson MA, Pallansch MA (2007) The complete genome sequence for an American isolate of enterovirus 77. Arch Virol 152:1587–1591

    Article  PubMed  CAS  Google Scholar 

  16. Salminen MO, Carr JK, Burke DS, McCutchan FE (1995) Identification of breakpoints in intergenotypic recombinants of HIV type 1 by bootscanning. AIDS Res Hum Retrovir 11:1423–1425

    Article  PubMed  CAS  Google Scholar 

  17. Smura T, Blomqvist S, Paananen A, Vuorinen T, Sobotová Z, Bubovica V, Ivanova O, Hovi T, Roivainen M (2007) Enterovirus surveillance reveals proposed new serotypes and provides new insight into enterovirus 5′-untranslated region evolution. J Gen Virol 88:2520–2526

    Article  PubMed  CAS  Google Scholar 

  18. Svitkin YV, Imataka H, Khaleghpour K, Kahvejian A, Liebig HD, Sonenberg N (2001) Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation. RNA 7:1743–1752

    Article  PubMed  CAS  Google Scholar 

  19. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  20. WHO (2004) Polio laboratory manual, 4th edn. World Health Oraganization/IVB/04.10. Geneva, Switzerland

  21. Yamashita T, Ito M, Tsuzuki H, Sakae K, Minagawa H (2010) Molecular identification of enteroviruses including two new types (EV-98 and EV-107) isolated from Japanese travellers from Asian countries. J Gen Virol 91:1063–1066

    Article  PubMed  CAS  Google Scholar 

  22. Zhang Y, Wang H, Zhu S, Li Y, Song L, Liu Y, Liu G, Nishimura Y, Chen L, Yan D, Wang D, An H, Shimizu H, Xu A, Xu W (2010) Characterization of a rare natural intertypic type 2/type 3 penta-recombinant vaccine-derived poliovirus isolated from a child with acute flaccid paralysis. J Gen Virol 91:421–429

    Article  PubMed  CAS  Google Scholar 

  23. Zhang Y, Zhang F, Zhu S, Chen L, Yan D, Wang D, Tang R, Zhu H, Hou X, An H, Zhang H, Xu W (2010) A Sabin 2-related poliovirus recombinant contains a homologous sequence of human enterovirus species C in the viral polymerase coding region. Arch Virol 155:197–205

    Article  PubMed  CAS  Google Scholar 

  24. Zhang Y, Wang J, Guo W, Wang H, Zhu S, Wang D, Bai R, Li X, Yan D, Wang H, Zhang Y, Zhu Z, Tan X, An H, Xu A, Xu W (2011) Emergence and transmission pathways of rapidly evolving evolutionary branch C4a strains of human enterovirus 71 in the Central Plain of China. PLoS One 6:e27895

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (project no. 30900063), the National Program on Key Basic Research Project (973 Program) (project no. 2011CB504902), and the National Key Technology R&D Program of China (project no. 2009ZX10004-202).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of Life Science, Shanxi University, Taiyuan City, Shanxi Province, People’s Republic of China

    Jitao Wang & Jianrong Han

  2. WHO WPRO Regional Polio Reference Laboratory, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping District, Beijing, 102206, People’s Republic of China

    Jitao Wang, Yong Zhang, Xiaolei Li, Shuangli Zhu, Dongmei Yan, Dongyan Wang, Hongqiu An & Wenbo Xu

  3. Taiyuan Center for Disease Control and Prevention, Taiyuan City, Shanxi Province, People’s Republic of China

    Jitao Wang

  4. Tibet Center for Disease Control and Prevention, Lhasa City, Tibet Autonomous Region, People’s Republic of China

    Mei Hong &  Tsewang

Authors
  1. Jitao Wang
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  2. Yong Zhang
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  3. Mei Hong
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  4. Xiaolei Li
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  5. Shuangli Zhu
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  6. Dongmei Yan
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  7. Dongyan Wang
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  8. Hongqiu An
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  9. Tsewang
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  10. Jianrong Han
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  11. Wenbo Xu
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Corresponding author

Correspondence to Wenbo Xu.

Additional information

J. Wang and Y. Zhang contributed equally to this work.

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Wang, J., Zhang, Y., Hong, M. et al. Isolation and characterization of a Chinese strain of human enterovirus 74 from a healthy child in the Tibet Autonomous Region of China. Arch Virol 157, 1593–1598 (2012). https://doi.org/10.1007/s00705-012-1332-9

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  • DOI: https://doi.org/10.1007/s00705-012-1332-9

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