Abstract
The complete genome sequence of a Chinese narcissus isolate of narcissus late season yellows virus from Zhangzhou, China (NLSYV-ZZ), was determined to be 9,651 nucleotides in length, excluding the 3′-terminal poly (A) tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,315 nucleotides encoding a polyprotein of 3,105 amino acids. The polyprotein was predicted to be cleaved into ten mature proteins by three viral proteases. Complete genome sequence comparison and phylogenetic analysis indicated that NLSYV-ZZ was most closely related to narcissus yellow stripe virus (NYSV), which was also isolated from narcissus. These viruses shared 69.9 % identity in their complete nucleotide sequences and 77.0 % identity in their polyprotein amino acid sequences.
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Acknowledgments
This study was jointly supported by research grants from Key Scientific and Technological Program of Fujian Province of China (No. 2009N0001), the Program of Fujian Entry-Exit Inspection and Quarantine Bureau of China (No. FK2011-07, FK2007-07), the natural Science Foundation of Fujian Province of China (No. 2009J01046) and the Program of Entry-Exit Inspection and Quarantine industry standard (No. 2010B320k, 2009B506k).
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Lin, SQ., Shen, JG., Gao, FL. et al. Complete genome sequence of narcissus late season yellows virus infecting Chinese narcissus in China. Arch Virol 157, 1821–1824 (2012). https://doi.org/10.1007/s00705-012-1328-5
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DOI: https://doi.org/10.1007/s00705-012-1328-5