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Analysis of deoxynucleosides in bacteriophages ϕEF24C and K and the frequency of a specific restriction site in the genomes of members of the bacteriophage subfamily Spounavirinae

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Abstract

The genera SPO1-like and Twort-like viruses in the subfamily Spounavirinae of the family Myoviridae have been newly proposed, with the reorganization of the SPO1-related bacteriophages (phages). A criterion defining these viral genera is the presence/absence of DNA modifications. In this study, liquid chromatography/mass spectrometry showed that phages ϕEF24C and K of the subfamily Spounavirinae have unmodified DNA, which classifies them as Twort-like viruses. Moreover, in the subfamily Spounavirinae, DNA modification and elimination of a particular DNA sequence were suggested to be the major antirestriction strategies of the SPO1-like and Twort-like viruses, respectively.

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Authors and Affiliations

  1. Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kohasu, Oko-cho, Nankoku City, Kochi, 783-8505, Japan

    Jumpei Uchiyama, Iyo Takemura, Shigenobu Matsuzaki & Masanori Daibata

  2. Division of Chemistry, Faculty of Education, Kochi University, Kochi, Japan

    Yusuke Maeda & Keiji Gamoh

Authors
  1. Jumpei Uchiyama
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  2. Yusuke Maeda
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  3. Iyo Takemura
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  4. Keiji Gamoh
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  5. Shigenobu Matsuzaki
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  6. Masanori Daibata
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Correspondence to Jumpei Uchiyama.

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J. Uchiyama and Y. Maeda contributed equally to this work.

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Uchiyama, J., Maeda, Y., Takemura, I. et al. Analysis of deoxynucleosides in bacteriophages ϕEF24C and K and the frequency of a specific restriction site in the genomes of members of the bacteriophage subfamily Spounavirinae . Arch Virol 157, 1587–1592 (2012). https://doi.org/10.1007/s00705-012-1324-9

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  • DOI: https://doi.org/10.1007/s00705-012-1324-9

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