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A large outbreak of enteritis in goat flocks in Marmara, Turkey, by G8P[1] group A rotaviruses

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Abstract

Group A rotaviruses are regarded as major enteric pathogens of large ruminants, while their impact on the health of small ruminants is not well documented. We report the detection of group A rotavirus from a large outbreak of enteritis that occurred in two goat flocks in the town of Kırklareli, Marmara Region, Turkey, in 2007. The disease was observed in young kids, with high morbidity and mortality rates, but not in adult animals. Rotavirus antigen was detected in the stools of the examined animals, and rotaviruses were isolated in MA104 cells. Upon sequencing of the VP4, VP6, VP7 and NSP4 genes, the strain (RVA/goat-tc/TUR/Kirklareli/2007/G8P[1]) was characterized as G8P[1], with E2 NSP4 and VP6 I2 genotype. These findings indicate that group A rotavirus should be included in the diagnostic algorithms for enteric disease in small ruminants.

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References

  1. Alkan F, Burgu I, Can-Sahna K, Cokcalıskan C (2004) Yeni dogan buzagı ishallerine karşı ticari ası ile asılanan sıgırlardan dogan yavrularda pasif bagışıklık duzeyi. Ankara Univ Vet Fak Derg 51:47–53

    Article  Google Scholar 

  2. Alkan F, Ozkul A, Oguzoglu TC, Timurkan MO, Calıskan E, Martella V, Burgu I (2010) Distribution of G (VP7) and P (VP4) genotypes of group A bovine rotaviruses from Turkish calves with diarrhea, 1997–2008. Vet Microbiol 141:231–237

    Article  PubMed  CAS  Google Scholar 

  3. Bányai K, Bogdán A, Szücs György, Arista S, De Grazia S, Kang G, Banerjee I, Iturriza-Gómara M, Buonavoglia C, Martella V (2009) Assignment of the group A rotavirus NSP4 gene into genotypes using a hemi-nested multiplex PCR assay: a rapid and reproducible assay for strain surveillance studies. J Med Microbiol 58:303–311

    Article  PubMed  Google Scholar 

  4. Can-Sahna K, Alkan F (2003) Sıgırlarda rotavirus enfeksiyonunun epidemiyolojisinde gebeligin rolu. Fırat Üniv Sağlık Bil Derg 17:203–209

    Google Scholar 

  5. Das BK, Gentsch JR, Cicirello HG, Woods PA, Gupta A, Ramachandran M, Kumar R, Bhan MK, Glass RI (1994) Characterization of rotavirus strains from newborn in New Delhi, India. J Clin Microbiol 32:1820–1822

    PubMed  CAS  Google Scholar 

  6. Estes M, Kapikian A (2007) Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (eds) Fields virology, 4th edn. Kluwer/Lippincott, Williams and Wilkins, Philadelphia, pp 1917–1974

    Google Scholar 

  7. Galindo-Cardiel I, Fernández-Jiménez M, Luján L, Buesa J, Espada J, Fantova E, Blanco J, Segalés J, Badiola JJ (2011) Novel group A rotavirus G8P[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs. Vet Microbiol 149:467–471

    Article  PubMed  CAS  Google Scholar 

  8. Gentsch JR, Glaa RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK (1992) Identification of Group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365–1373

    PubMed  CAS  Google Scholar 

  9. Ghosh S, Alam MM, Ahmed MU, Talukdar RI, Paul SK, Kobayashi N (2010) The complete genome constellation of a caprine group A rotavirus strains reveals common evolution with ruminant and human rotavirus strains. J Gen Virol 91:2367–2373

    Article  PubMed  CAS  Google Scholar 

  10. Ghosh S, Gatheru Z, Nyangao J, Adachi N, Urushibari N, Kobayashi N (2011) Full genomic analysis of a G8P[1] rotavirus strain isolated from an asymptomatic infant in Kenya provides evidence for an artiodactyl-to-human interspecies transmission event. J Med Virol 83:367–376

    Article  PubMed  Google Scholar 

  11. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY (1990) Polymerase chain reaction amplification and typing of rotavirus nucleic acids from stool specimens. J Clin Microbiol 28:276–282

    PubMed  CAS  Google Scholar 

  12. Gouvea V, Santos N, Timenetsky MC (1994) Identification of bovine and porcine rotavirus G types by PCR. J Clin Microbiol 32:1338–1340

    PubMed  CAS  Google Scholar 

  13. Gulati RB, Nakagomi O, Koshimura Y, Nakagomi T, Pandey R (1999) Relative frequencies of G and P types among rotavirus from Indian diarrheic cow and buffalo calves. J Clin Microbiol 37:2074–2076

    PubMed  CAS  Google Scholar 

  14. Gulati BR, Deepa R, Singh BK, Rao CD (2007) Diversity in Indian equine rotaviruses: identification of G10, P6 and G1 type and a new VP7 genotype (G16) strains in diarrheic foals in India. J Clin Microbiol 45:972–978

    Article  PubMed  CAS  Google Scholar 

  15. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98

    CAS  Google Scholar 

  16. Iturriza-Gomara M, Green J, Brown DW, Desselberger U, Gray JJ (1999) Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses. J Virol Methods 78:93–103

    Article  PubMed  CAS  Google Scholar 

  17. Iturriza Gómara M, Wong C, Blome S, Desselberger U, Gray J (2002) Molecular characterization of VP6 genes of human rotavirus isolates: correlation of genogroups with subgroups and evidence of independent segregation. J Virol 76:6596–6601

    Article  PubMed  Google Scholar 

  18. Kaminjolo JS, Adesiyun AA (1994) Rotavirus infection in calves, piglets, lambs and goat kids in Trinidad. Br Vet J 150:293–299

    Article  PubMed  CAS  Google Scholar 

  19. Lee JB, Joun SJ, Nakagomi T, Park SY, Kim TJ, Song CS, Jang HK, Kim BS, Nakagomi O (2003) Isolation, serologic and molecular characterization of the first G3 caprine rotavirus. Arch Virol 148:643–657

    Article  PubMed  CAS  Google Scholar 

  20. Martella V, Ciarlet M, Baselga R, Arista S, Elia G, Lorusso E, Banyai K, Terio V, Madio A, Ruggeri FM, Falcone E, Camero M, Decaro N, Buonavoglia C (2005) Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotavirus, sharing a common evolutionary origin with human G2 rotavirus. Virology 337:111–123

    Article  PubMed  CAS  Google Scholar 

  21. Martella V, Ciarlet M, Banyai K, Lorusso E, Cavalli A, Corrente M, Elia G, Arista S, Camero M, Desario C, Decaro N, Lavazza A, Buonavoglia C (2006) Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346:301–311

    Article  PubMed  CAS  Google Scholar 

  22. Martella V, Ciarlet M, Banyai K, Lorusso E, Arista S, Lavazza A, Pezzotti G, Decaro N, Cavalli A, Lucente MS, Corrente M, Elia G, Camero M, Tempesta M, Buonavoglia C (2007) Identification of group A porcine rotavirus strains bearing a novel VP4 (P) genotype in Italian swine herds. J Clin Microbiol 45:577–580

    Article  PubMed  CAS  Google Scholar 

  23. Martella V, Banyai K, Matthijnssens J, Buonavoglia C, Ciarlet M (2010) Zoonotic aspects of rotaviruses. Vet Microbiol 140:246–255

    Article  PubMed  CAS  Google Scholar 

  24. Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gómara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82:3204–3219

    Article  PubMed  CAS  Google Scholar 

  25. Matthijnssens J, Potgieter CA, Ciarlet M, Parreňo V, Martella V, Banyai K, Garaicoechea L, Palombo EA, Novo L, Zeller M, Arista S, Gerna G, Rahman M, Van Ranst M (2009) Are human P[14] Rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla? J Virol 83:2917–2929

    Article  PubMed  CAS  Google Scholar 

  26. Matthijnssens J, Ciarlet M, Mc Donald SM, Attoui H, Banyai K et al (2011) Uniformity of rotavirus strain nomenclature proosed by the rotavirus classification working group (RCWG). Arch Virol 156:1397–1413

    Article  PubMed  CAS  Google Scholar 

  27. Mc Neal MM, Sesta K, Choi AH, Basu M, Cole MJ, Aye PP, Bohm RP, Ward RL (2005) Development of a rotavirus-shedding model in rhesus macaques, using a homologous wild-type rotavirus of a new P genotype. J Virol 79:944–954

    Article  CAS  Google Scholar 

  28. Mendes VM, De Beer MC, Goosen GH, Steele AD (1994) Isolation and preliminary characterization of a caprine rotavirus. Onderstepoort J Vet Res 61:291–294

    PubMed  CAS  Google Scholar 

  29. Muňoz M, Alverez M, Lanza I, Cármenes P (1996) Role of enteric pathogens in the aetiology of neonatal diarrhoea in lambs and goat kids in Spain. Epidemiol Infect 117:203–211

    Article  PubMed  Google Scholar 

  30. Ozkul A, Yesilbag K, Karaoglu T, Burgu I (2002) Elecrophoretypes of bovine rotavirus detected in Turkey. Turk J Vet Anim Sci 26:359–362

    Google Scholar 

  31. Pratelli A, Martella V, Tempesta M, Buonavoglia C (1999) Characterization by polymerase chain reaction of ruminant rotaviruses isolated in Italy. New Microbiol 22:105–109

    PubMed  CAS  Google Scholar 

  32. Rahman M, Matthijnssens J, Nahar S, Podder G, Sack DA, Azim T, Van Ranst M (2005) Characterization of a novel P[25], G11 human group a rotavirus. J Clin Microbiol 43:3208–3212

    Article  PubMed  CAS  Google Scholar 

  33. Rao CD, Gowda K, Reddy BSY (2000) Sequence analysis of VP4 and VP7 genes of nontypeable strains identifies a new pair of outer capsid proteins representing novel P and G genotypes in bovine rotaviruses. Virology 276:104–113

    Article  PubMed  CAS  Google Scholar 

  34. Reidy N, Lennon G, Fanning S, Power E, O’Shea H (2006) Molecular characterization and analysis of bovine rotavirus strains circulating in Ireland 2002–2004. Vet Microbiol 117:242–247

    Article  PubMed  CAS  Google Scholar 

  35. Scott AC, Luddington J, Lucas M, Gilbert FR (1978) Rotavirus in goat. Vet Rec 103:145

    Article  PubMed  CAS  Google Scholar 

  36. Steyer A, Poljsak-Prijatelj M, Barlic-Maganja D, Jamnikar U, Mijovski JZ, Marin J (2007) Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia. Virology 359:275–282

    Article  PubMed  CAS  Google Scholar 

  37. Takahashi E, Inaba Y, Sato K, Kurogi H, Akashi H, Satoda K, Omori T (1979) Antibody to rotavirus in various animal species. Natl Inst Anim Health Q (Tokyo) 19:72–73

    CAS  Google Scholar 

  38. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA 5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol and Evol 28:2731–2739

    Article  CAS  Google Scholar 

  39. Wani SA, Bhot MA, Nawchoo R, Munshi ZHX, Bach AS (2004) Evidence of Rotavirus associated with Neonatal lamb Diarrhoea in India. Trop Anim Health Prod 36:27–32

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Virology Department, Faculty of Veterinary Medicine, Ankara University, Diskapi, Ankara, 06110, Turkey

    Feray Alkan

  2. Central Veterinary Control and Research Institute, Pendik, Istanbul, Turkey

    Veli Gulyaz, Selma Iyisan, Selma Ozdemir & Nesrin Turan

  3. Virology Department, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey

    M. Ozkan Timurkan

  4. Department of Veterinary Public Health, University of Bari, Bari, Italy

    Canio Buonavoglia & Vito Martella

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  1. Feray Alkan
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  2. Veli Gulyaz
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Correspondence to Feray Alkan.

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Alkan, F., Gulyaz, V., Ozkan Timurkan, M. et al. A large outbreak of enteritis in goat flocks in Marmara, Turkey, by G8P[1] group A rotaviruses. Arch Virol 157, 1183–1187 (2012). https://doi.org/10.1007/s00705-012-1263-5

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