Abstract
Two co-infecting novel vitiviruses from Actinidia chinensis were identified from mechanically inoculated Nicotiana occidentalis. Both virus genomes were sequenced and share 64% nucleotide identity. Their overall structure is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3′ end. Open reading frame 4 (ORF4) encodes the coat protein, the most conserved gene of the vitiviruses, in which they share 75% amino acid identity, 61-68% with grapevine virus B, 55-59% with grapevine virus A, and 37-42% with grapevine virus E. Based on the molecular criteria for species demarcation in the family Betaflexiviridae, these are two novel viruses, tentatively named Actinidia virus A and Actinidia virus B.
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Acknowledgements
This research was supported partially by the Foundation for Research, Science and Technology, New Zealand (C06X0710), and capability funding from The New Zealand Institute for Plant & Food Research Ltd. The authors would like to thank Donald Hunter for his valuable suggestions on this manuscript and Gardette Valmonte for her help with phylogenetic analysis.
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Blouin, A.G., Chavan, R.R., Pearson, M.N. et al. Detection and characterisation of two novel vitiviruses infecting Actinidia . Arch Virol 157, 713–722 (2012). https://doi.org/10.1007/s00705-011-1219-1
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DOI: https://doi.org/10.1007/s00705-011-1219-1