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Seroprevalence of porcine cytomegalovirus and sapovirus infection in pigs in Hunan province, China

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Abstract

The seroprevalence of porcine cytomegalovirus (PCMV) and sapovirus (SaV) infections in pigs was investigated in Hunan province, China, between May 2005 and October 2010. A total of 500 pig serum samples collected from 10 representative administrative regions in Hunan province were evaluated for antibodies against PCMV and SaV using enzyme-linked immunosorbent assay (ELISA). The overall seroprevalence of porcine cytomegalovirus and sapovirus in pigs was 96.40% (482/500) and 63.40% (317/500), and the seropositivity of 10 herds we surveyed varied, ranging from 94.74% to 98.48% and 56.36% to 72.50%, respectively. The highest prevalence was found in breeding sows (96.67% for PCMV and 83.33% for SaVs). The results of the present survey indicated that infections with porcine cytomegalovirus and sapovirus are highly prevalent in pigs in Hunan province, China.

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References

  1. Alcalá AC, Rodríguez-Díaz J, de Rolo M, Vizzi E, Buesa J, Liprandi F, Ludert JE (2010) Seroepidemiology of porcine enteric sapovirus in pig farms in Venezuela. Vet Immunol Immunopathol 137:269–274

    Article  PubMed  Google Scholar 

  2. Anderson EJ (2010) Prevention and treatment of viral diarrhea in pediatrics. Expert Rev Anti Infect Ther 8:205–217

    Article  PubMed  Google Scholar 

  3. Assaf R, Boulillant AMP, Franco ED (1982) Enzyme linked immunosorbent assay for the detection of antibodies to porcine cytomegalovirus. Can Com Med 46:183–185

    CAS  Google Scholar 

  4. Collett MG, Roberts DC (2002) Cytomegalovirus infection in a pig in South Africa. J S Afr Vet Assoc 73:44–46

    PubMed  CAS  Google Scholar 

  5. Cunha JB, de Mendonça MC, Miagostovich MP, Leite JP (2010) Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil. Arch Virol 155:1301–1305

    Article  PubMed  CAS  Google Scholar 

  6. Edington N, Watt RG, Plowright W (1977) Experimental transplacental transmission of porcine cytomegalovirus. J Hyg (Lond) 78:243–251

    Article  CAS  Google Scholar 

  7. Fishman JA, Rubin RH (1998) Infection in organ transplant recipients. N Engl J Med 338:1741–1751

    Article  PubMed  CAS  Google Scholar 

  8. Goltz M, Widen F, Banks M, Belak S, Ehlers B (2000) Characterization of the DNA Polymerase Loci of Porcine Cytomegaloviruses from Diverse Geographic Origins. Virus Genes 21:249–255

    Article  PubMed  CAS  Google Scholar 

  9. Guo M, Hayes J, Cho KO, Parwani AV, Lucas LM, Saif LJ (2001) Comparative pathogenesis of tissue culture-adapted and wild-type Cowden porcine enteric calicivirus (PEC) in gnotobiotic pigs and induction of diarrhea by intravenous inoculation of wild-type PEC. J Virol 75:9239–9251

    Article  PubMed  CAS  Google Scholar 

  10. Guo M, Qian Y, Chang KO, Saif LJ (2001) Expression and self-assembly in baculovirus of porcine enteric calicivirus capsids into virus-like particles and their use in an enzyme-linked immunosorbent assay for antibody detection in swine. J Clin Microbiol 39:1487–1493

    Article  PubMed  CAS  Google Scholar 

  11. Hamel AL, Lin L, Sachvie C, Grudeski E, Nayar GP (1999) PCR assay for detecting porcine cytomegalovirus. J Clin Microbiol 37:3767–3768

    PubMed  CAS  Google Scholar 

  12. Hamprecht K, Goelz R, Maschmann J (2005) Breast milk and cytomegalovirus infection in preterm infants. Early Hum Dev 81(12):989–996 Epub 2005 Nov 7

    Article  PubMed  CAS  Google Scholar 

  13. Jin M, Yu JM, Li HY, Zang Q, Cui SX (2010) Genetic diversity of porcine sapoviruses from Lulong County in China. Chin J Virol 26:255–259 (in Chinese)

    CAS  Google Scholar 

  14. Lee CS, Moon HJ, Yang JS, Park SJ, Song DS, Kang BK, Park BK (2007) Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. J Virol Methods 139:39–43

    Article  PubMed  CAS  Google Scholar 

  15. L’Homme Y, Brassard J, Ouardani M, Gagné MJ (2010) Characterization of novel porcine sapoviruses. Arch Virol 155:839–846

    Article  PubMed  Google Scholar 

  16. Li J, Yu XL, Li RC, Luo W, Xiang WJ, Li W, Wang Y, Ge M (2009) PCR detection and sequence analysis of gB gene of porcine cytomegalovirus. J Hunan Agri Univ (Nat Sci) 35:521–525 (in Chinese)

    CAS  Google Scholar 

  17. Li J, Yu XL, Li RC, Huang ZB, Ge M, Xiang WJ, Li W, Wang Y (2010) Expression and antigenic analysis of the immunodominant region of porcine cytomegalovirus gB protein. Chin J Prev Vet Med 2:126–130 (in Chinese)

    Google Scholar 

  18. Li RC, Liu GH, Huang ZB, Ding J, Wang ZN, Xiao CT, Yu XL (2011) Expression of VP1 gene and ELISA for detection of antibodies against porcine sapoviruses. Chin Vet Sci (in press) (in Chinese)

  19. Liu XC, Yin YB, Zhang Y, Xiu SZ, Hai LM, Wang X (2009) Development of PCR assay for porcine cytomegalovirus detection. Chin J Vet Med 45:34–35 (in Chinese)

    Google Scholar 

  20. Lopo S, Vinagre E, Palminha P, Paixao MT, Nogueira P, Freitas MG (2011) Seroprevalence to cytomegalovirus in the Portuguese population, 2002–2003. Euro Surveill 16(25) (pii:19896)

    Google Scholar 

  21. Mijovski JZ, Poljsak-Prijatelj M, Steyer A, Barlic-Maganja D, Koren S (2010) Detection and molecular characterisation of noroviruses and sapoviruses in asymptomatic swine and cattle in Slovenian farms. Infect Genet Evol 10:413–420

    Article  PubMed  CAS  Google Scholar 

  22. Phan TG, Trinh QD, Yagyu F, Okitsu S, Ushijima H (2007) Emergence of rare sapovirus genotype among infants and children with acute gastroenteritis in Japan. Eur J Clin Microbiol Infect Dis 26:21–27

    Article  PubMed  CAS  Google Scholar 

  23. Prichard MN, Kern ER (2011) The search for new therapies for human cytomegalovirus infections. Virus Res 157:212–221

    Article  PubMed  CAS  Google Scholar 

  24. Rondhuis PR, De JMF, Sdhep J (1980) Indirect fluorescence antibody studies of porcine cytomegalo virus infections in the Netherlands. Tjidschr Diergeneeskd 105:46–58

    Google Scholar 

  25. Shen Q, Zhang W, Yang S, Chen Y, Ning H, Shan T, Liu J, Yang Z, Cui L, Zhu J, Hua X (2009) Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009. Arch Virol 154:1625–1630

    Article  PubMed  CAS  Google Scholar 

  26. Sung H, Schleiss MR (2010) Update on the current status of cytomegalovirus vaccines. Expert Rev Vaccines 9:1303–1314

    Article  PubMed  CAS  Google Scholar 

  27. Tajima T, Hironao T, Kajikawa T, Kawamura H (1993) Application of enzyme-link immunosorbent assay for the seroepizootiological survey of antibodies against porcine cytomegalovirus. J Vet Med Sci 55:421–424

    Article  PubMed  CAS  Google Scholar 

  28. Yang S, Zhang W, Shen Q, Huang F, Wang Y, Zhu J, Cui L, Yang Z, Hua X (2009) Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine. Virol J 6:216

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant no. 30571390) and the Science and Technology Foundation of Hunan Province (grant no. 2007FJ1003).

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Correspondence to Xing-Long Yu.

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Liu, GH., Li, RC., Li, J. et al. Seroprevalence of porcine cytomegalovirus and sapovirus infection in pigs in Hunan province, China. Arch Virol 157, 521–524 (2012). https://doi.org/10.1007/s00705-011-1189-3

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  • DOI: https://doi.org/10.1007/s00705-011-1189-3

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