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Phenotypic and genotypic characterization of dengue virus isolates differentiates dengue fever and dengue hemorrhagic fever from dengue shock syndrome

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Abstract

Dengue viruses (DENV) cause 50-100 million cases of acute febrile disease every year, including 500,000 reported cases of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Viral factors have been proposed to influence the severity of the disease, but markers of virulence have never been identified on DENV. Three DENV serotype-1 isolates from the 2007 epidemic in Cambodia that are derived from patients experiencing the various clinical forms of dengue were characterized both phenotypically and genetically. Phenotypic characteristics in vitro, based on replication kinetics in different cell lines and apoptosis response, grouped isolates from DF and DHF patients together, whereas the virus isolate from a DSS patient showed unique features: a lower level of replication in mammalian cells and extensive apoptosis in mosquito cells. Genomic comparison of viruses revealed six unique amino acid residues in the membrane, envelope, and in non-structural genes in the virus isolated from the DSS patient.

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Acknowledgments

This work was supported by the French Armed Forces Medical Service, the French Direction Générale de l’Armement (contrat d’objectif 07co405) and from the European Union (DENFRAME project, 6th Framework Programme: 2005-INCO-DEV2-n°517711).

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Authors and Affiliations

  1. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

    Anne Tuiskunen, Maria Wahlström & Åke Lundkvist

  2. Swedish Institute for Communicable Disease Control, Solna, Sweden

    Anne Tuiskunen & Åke Lundkvist

  3. Virology Department, French Armed Forces Biomedical Research Institute (IRBA), Marseille, France

    Anne Tuiskunen, Vanessa Monteil, Sébastien Plumet, Laetitia Boubis, Hugues Tolou & Isabelle Leparc-Goffart

  4. Virology Unit, Institut Pasteur in Cambodia, Phnom Penh, Cambodia

    Veasna Duong & Philippe Buchy

  5. Unité de Virologie Tropicale, Institut de Recherche Biomédicale des Armées, allée du Médecin Colonel Eugène Jamot, Parc du Pharo, BP60109, 13262, Marseille Cedex 07, France

    Isabelle Leparc-Goffart

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  1. Anne Tuiskunen
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  2. Vanessa Monteil
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  3. Sébastien Plumet
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  10. Isabelle Leparc-Goffart
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Correspondence to Isabelle Leparc-Goffart.

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Tuiskunen, A., Monteil, V., Plumet, S. et al. Phenotypic and genotypic characterization of dengue virus isolates differentiates dengue fever and dengue hemorrhagic fever from dengue shock syndrome. Arch Virol 156, 2023–2032 (2011). https://doi.org/10.1007/s00705-011-1100-2

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  • DOI: https://doi.org/10.1007/s00705-011-1100-2

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