Abstract
Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in the United States.
Similar content being viewed by others
References
Argüello-Astorga GR, Guevara-González RG, Herrera-Estrella LR, Rivera-Bustamante RF (1994) Geminivirus replication origins have a group specific organisation of iterative elements: a model for replication. Virology 203:90–100
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Echemendía AL, Ramos PL, Díaz Peral L, Fuentes A, Pujol M, González G (2004) First report of Sida golden yellow vein virus infecting Sida spp. in Cuba. Plant Pathol 53:234
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821
Fontes EPB, Eagle PA, Sipe PS, Luckow VA, Hanley-Bowdoin L (1994) Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. J Biol Chem 269:8459–8465
Graham AP, Martin DP, Roye ME (2010) Molecular characterisation and phylogeny of two begomoviruses infecting Malvastrum americanum in Jamaica: evidence of the contribution of inter-species recombination to the evolution of malvaceous weed-associated begomoviruses from the Northern Caribbean. Virus Genes 40:256–266
Graham AP, Stewart CS, Roye ME (2007) First report of a begomovirus infecting two common weeds: Malvastrum americanum and Sida spinosa in Jamaica. Plant Pathol 56:340
Levy JA, Fraenkel-Conrat H, Owens RA (1999) Virology, 3rd edn. Prentice-Hall Inc., Upper Saddle River
McGlashan D, Polston JE, Bois D (1994) Tomato leaf curl geminivirus in Jamaica. Plant Dis 78:1219
Morales FJ, Anderson PK (2001) The emergence and dissemination of whitefly-transmitted geminiviruses in Latin America. Arch Virol 146:415–441
Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347
Roye ME, Collins AM, Maxwell DP (2006) First report of a begomovirus associated with the common weed Jatropha gossypifolia in Jamaica. Plant Pathol 55:286–286
Roye ME, Brown S, Spence JD, Smith KN, McLaughlin WA, Brown JK, Maxwell DP (2003) Ten years of molecular biology research on whitefly transmitted geminivirus from Jamaica: a review. Jam J Sci Technol 14:98–118
Roye ME, Wernecke ME, McLaughlin WA, Nakhla MK, Maxwell DP (1999) Tomato dwarf leaf curl virus, a new bipartite geminivirus associated with tomatoes and peppers in Jamaica and mixed infection with tomato leaf curl virus. Plant Pathol 48:370–378
Roye ME, McLaughlin WA, Nakhla MK, Maxwell DP (1997) Genetic diversity among geminiviruses associated with the weed species Sida spp., Macroptilium lathyroides and Wissadula amplissima from Jamaica. Plant Dis 81:1251–1258
Smith KN (2005) The molecular characterisation of the begomoviruses in cabbage in Jamaica. MPhil thesis, University of the West Indies, Mona
Thresh JM, Otim-Nape GW, Thankappan M, Muniyappa V (1998) The mosaic diseases of cassava in Africa and India caused by whitefly-borne geminiviruses. Rev Plant Pathol 77:935–945
Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164
Acknowledgments
This work was funded by the School of Graduate Studies and Research and the Principal’s New Initiative Fund, UWI, Mona, and the Department of Plant Pathology, UCD.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Stewart, C.S., Kon, T., Gilbertson, R.L. et al. First report of the complete sequence of Sida golden yellow vein virus from Jamaica. Arch Virol 156, 1481–1484 (2011). https://doi.org/10.1007/s00705-011-1030-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-011-1030-z