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Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010

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Abstract

A/equine/Kanazawa/1/2007 (H3N8), A/equine/Hokkaido/I828/2008 (H3N8) and A/equine/Mongolia/1/2008 (H3N8) were isolated from infected horses. A/equine/Yokohama/aq19/2009 (H3N8) and A/equine/Yokohama/aq13/2010 (H3N8) were isolated from horses imported from Canada and Belgium examined at the Animal Quarantine Service in Yokohama, Japan. In the present study, these five isolates were genetically and antigenically analyzed. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed that three isolates from horses in Japan and imported from Canada belonged to the same branch, clade 1 of the Florida sublineage, while the isolates from horses in Mongolia and imported from Belgium belonged to another branch, clade 2 of the Florida sublineage. Reactivity patterns of a panel of monoclonal antibodies to the HA of A/equine/Kanazawa/1/2007 (H3N8) with the five isolates indicate that the HAs of these viruses were antigenically similar to each other and to the reference strains A/equine/La Plata/1/1993 (H3N8) and A/equine/Avesta/1/1993 (H3N8). The present findings indicate that extensive antigenic variation has not accumulated among H3N8 influenza viruses in horses.

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Acknowledgments

We thank Dr. Jin-Sik Oh (Binote Inc., Korea) and Dr. Hiroaki Wakamoto (Chisso Corp., Japan) for helping to produce MAbs to Eq/Kanazawa/07.

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, 060-0818, Japan

    Masayuki Motoshima, Masatoshi Okamatsu, Shingo Asakura, Saya Kuribayashi, Yoshihiro Sakoda & Hiroshi Kida

  2. State Central Veterinary Laboratory, Ulaanbaatar, 210153, Mongolia

    Sugar Sengee, Damdinjav Batchuluun & Ruuragchaa Sodnomdarjaa

  3. Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, 920-3101, Japan

    Mika Ito

  4. Ishikari Livestock Hygiene Service Center, Sapporo, 062-0045, Japan

    Yukiko Maeda

  5. Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, 235-0008, Japan

    Mariko Eto

  6. Research Center for Zoonosis Control, Hokkaido University, Sapporo, 001-0021, Japan

    Hiroshi Kida

Authors
  1. Masayuki Motoshima
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  2. Masatoshi Okamatsu
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  3. Shingo Asakura
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  4. Saya Kuribayashi
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  5. Sugar Sengee
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  7. Mika Ito
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  8. Yukiko Maeda
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  9. Mariko Eto
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  10. Yoshihiro Sakoda
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  11. Ruuragchaa Sodnomdarjaa
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  12. Hiroshi Kida
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Correspondence to Hiroshi Kida.

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Motoshima, M., Okamatsu, M., Asakura, S. et al. Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010. Arch Virol 156, 1379–1385 (2011). https://doi.org/10.1007/s00705-011-1000-5

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  • DOI: https://doi.org/10.1007/s00705-011-1000-5

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