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Genotyping of mumps virus circulating in Turkey in the 2006–2007 winter season

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Abstract

In the winter of 2006–2007, several parotitis cases were reported in different provinces of Turkey. Serological and virological studies were undertaken to investigate these cases with the aim of determining the genotype of the mumps virus (MuV) circulating in Turkey. Samples from 23 cases—Ankara (n:5), Kırklareli (n:4), Mugla (n:10), Isparta (n:3), Trabzon (n:1)—with a diagnosis of clinical parotitis were investigated. Serum samples were tested against mumps IgM and IgG, nested PCR amplification of a 639-bp fragment encompassing the entire SH gene was performed using buccal swabs, and PCR products were sequenced. Of 18 serum samples, 16 (88.9%) were positive for mumps IgM. Seven (30.4%) of 23 buccal swab samples were positive by PCR. In five PCR-positive cases, the sample was also positive for mumps IgM, and serum samples were not available from two of the PCR-positive cases. There was 98% identity between the different sequences, and all were identified as genotype H. The sequences were most similar to sequences identified in Spain, Japan, Switzerland and the UK, and less closely related to the H strains identified in Belarus, Korea and Russia. This is the first report of the mumps virus genotypes circulating in Turkey. Turkey is, geographically, a bridge between Europe and Asia, and therefore, a better understanding of the molecular epidemiology of MuV in Turkey may led to improved tracking of the circulation of strains between the two continents. Moreover, there is a need to further investigate the existence of other genotypes in Turkey.

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Acknowledgments

We would like to thank Drs. Kevin Brown and Li Jin, Virus Reference Department, Centre for Infection, HPA, London, for their help in sequencing and genotyping the isolates, Dr. Etem Ozkaya for evaluation of the sequence data, the laboratory personnel of RSNPHA of Turkey for excellent technical assistance, and the staff of the Primary Health Care directorate of the Turkish Ministry of Health, Provincial Health Directorates, for organising the sample collection and collaboration.

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  1. Alper Akcali

    Present address: Department of Microbiology and Clinical Microbiology, School of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Authors and Affiliations

  1. Virology Reference and Research Laboratory, Refik Saydam National Public Health Agency (RSNPHA), Sihhiye, 06100, Ankara, Turkey

    Alper Akcali, Neziha Yilmaz & Yavuz Uyar

  2. Presidency, Refik Saydam National Public Health Agency (RSNPHA), Sihhiye, 06100, Ankara, Turkey

    Mustafa Ertek

  3. Ministry of Health of Turkey, Sihhiye, 06100, Ankara, Turkey

    Turan Buzgan

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  1. Alper Akcali
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  2. Neziha Yilmaz
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Correspondence to Alper Akcali.

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Akcali, A., Yilmaz, N., Uyar, Y. et al. Genotyping of mumps virus circulating in Turkey in the 2006–2007 winter season. Arch Virol 154, 1807–1812 (2009). https://doi.org/10.1007/s00705-009-0519-1

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  • DOI: https://doi.org/10.1007/s00705-009-0519-1

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