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A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus)

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Abstract

Electron microscopy of extracts from diseased Polygonum convolvulus plants from Piedmont (Italy) revealed particles with the morphological features of a tospovirus. Sequencing of the full-length small (S) and medium (M) genome segments indicated that the virus is a member of a new Tospovirus species provisionally named Polygonum ringspot virus. A feature distinguishing it from members of other Tospovirus species was the presence of a very short intergenic region on the S segment lacking the potential for formation of the predicted hairpin structure involved in subgenomic expression. Antibodies made against purified nucleocapsids allowed serological comparison with other tospovirus isolates and revealed a relationship with tomato yellow ring virus, and to a lesser extent, to iris yellow spot virus. Serological tests detected the virus in various locations in northern and central Italy. The experimental host range was wide, although in nature the virus appeared restricted to two Polygonum species.

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References

  1. Bucher E, Sijen T, de Haan P, Goldbach R, Prins M (2003) Negative-strand tospoviruses carry a gene for gene silencing at analogous genomic positions. J Virol 77:1329–1336

    Article  PubMed  CAS  Google Scholar 

  2. Cortes I, Livieratos IC, Derks A, Peters D, Kormelink R (1998) Molecular and serological characterization of Iris yellow spot virus, a new and distinct Tospovirus species. Phytopathology 88:1276–1282

    Article  PubMed  CAS  Google Scholar 

  3. Cortes I, Saajer J, Wongjkaew KS, Pereire AM, Goldbach R, Peters D, Kormelink R (2001) Identification and characterization of a novel Tospovirus species using RT-PCR approach. Arch Virol 146:265–278

    Article  Google Scholar 

  4. Cosmi T, Marchesini E, Martini G (2003) Presenza e diffusione di Tospovirus e di tripidi vettori in Veneto. L’Informatore Agrario 20:69–72

    Google Scholar 

  5. De Avila AC, De Haan P, Kormelink DO, Resende R, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J Gen Virol 74:153–159

    Article  PubMed  Google Scholar 

  6. De Avila AC, De Haan P, Smeets MLL, Resende R, Kormelink DO, Kitajima EW, Goldbach RW, Peters D (1993) Distinct levels of relationships between tospovirus isolates. Arch Virol 128:211–227

    Article  PubMed  Google Scholar 

  7. Felsenstein J (1989) PHYLIP-phylogeny inference package (version 3.2). Cladistics 5:164–166

    Google Scholar 

  8. Fauquet CM, Mayo MA, Maniloff J, DesselbergerU, Ball LA (2005) Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Amsterdam, pp 712–716

  9. Gera A, Chen J, Salomon R, Raccah B (1998) Iris yellow spot tospovirus detected in onion (Allium cepa) in Israel. Plant Dis 82:127

    Article  Google Scholar 

  10. Ghotbi T, Shahraeen N, Winter S (2005) Occurrence of Tospovirus in ornamental and weed species in Markazi and Tehran Provinces in Iran. Plant Dis 89:425–429

    Article  Google Scholar 

  11. Giorgi C, Accardi L, Nicoletti L, Gro MC, Takehara K, Hilditch C, Morikawa S, Bishop DHL (1991) Sequences and coding strategies of the S RNA of Toscana and Rift valley fever viruses compared to those of Punta toro, Sicilian sandfly fever, and Uukuniemi viruses. Virology 180:738–753

    Article  PubMed  CAS  Google Scholar 

  12. Goldbach R, Kuo G (1996) Introduction: proceedings of the international symposium on tospovirus and thrips of floral and vegetable crops. Acta Hortic 431:21–26

    Google Scholar 

  13. Goldbach R, Peters D (1996) Molecular and biological aspects of Tospoviruses. In: Elliott RM (ed) The Bunyaviridae. Plenum Press, New York, pp 129–157

  14. Hassani-Mehraban A, Saijer J, Peters D, Goldbach R, Kormelink R (2005) A new tomato-infecting tospovirus from Iran. Phytopathology 95:852–858

    Article  PubMed  CAS  Google Scholar 

  15. Hassani-Mehraban A, Saijer J, Peters D, Goldbach R, Kormelink R (2007) Molecular and biological comparison of two tomato yellow ring (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol 152:85–96

    Article  PubMed  CAS  Google Scholar 

  16. Heydarnejad J, Barclay WS, Izadpanah K, Hunter FR, Gooding MJ (2006) Molecular characterization of Iranian wheat stripe virus shows its taxonomic position as a distinct species in the genus Tenuivirus. Arch Virol 151:217–227

    Article  PubMed  CAS  Google Scholar 

  17. Jones DR (2005) Plant viruses transmitted by thrips. Eur J Plant Path 113:119–157

    Article  Google Scholar 

  18. van Knippernberg I, Goldbach R, Kormelink R (2005) Tomato spotted wilt virus S-segment mRNA have overlapping 3′-end containing a predicted stem loop structure and conserved sequence motif. Virus Res 110:125–131

    Article  Google Scholar 

  19. Marullo R, zur Strassen R (2003) Checklist of the species of the Italian Fauna: Thysanoptera. Minelli A, Ruffo S, La Posta S (eds) On-line version 2.0. http://www.faunaitalia.it/checklist

  20. Moyer JW (1999) Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic press, New York, pp 1803–1807

    Google Scholar 

  21. Mavric I, Ravnikar M (2001) Iris yellow spot tospovirus in Slovenia. In: Catara A, Albanese G and Catara (eds) Proceedings of the 5th congress of the European foundation for plant pathology: biodiversity in plant pathology, Taormina-Giardini Naxos, Italy, 18–22, September 2000, pp 223–225

  22. Mumford RA, Barker I, Wood KR (1996) The biology of the tospoviruses. Ann of Applied Biol 128:159–183

    Article  Google Scholar 

  23. Mumford RA, Jarvis B, Morris J, Blockley A (2003) First report of Chrysanthemum stem necrosis virus (CSNV) in the U.K. Plant Path 52:779

    Article  Google Scholar 

  24. Nathanson N, Gonzàles-Scarano F (1999) Bunyaviridae, General Features. In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic press, New York, pp 204–212

    Google Scholar 

  25. Nguyen M, Haenni AL (2003) Expression strategies of ambisense viruses. Virus Res 93:141–150

    Article  PubMed  CAS  Google Scholar 

  26. Ohnishi J, Katsuzaki H, Tsuda S, Sakurai T, Murai T (2006) Frankliniella cephalica, a new vector for Tomato spotted wilt virus. Plant Dis 90:685

    Article  Google Scholar 

  27. Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comp Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  28. Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (Family Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs. Arch Virol 145:1035–1045

    Article  PubMed  CAS  Google Scholar 

  29. Rasoulpour R, Izadpanah K (2007) Characterization of cineraria strain of tomato yellow ring virus from Iran. Australas Plant Pathol 36:286–294

    Article  CAS  Google Scholar 

  30. Roggero P, Ciuffo M, Vaira AM, Accotto GP, Masenga V, Milne RG (2000) An ophiovirus isolated from lettuce with big-vein symptoms. Arch Virol 145:2629–2642

    Article  PubMed  CAS  Google Scholar 

  31. Sakurai T, Inoue T, Tsuda S (2004) Distinct efficiencies of Impatiens necrotic spot virus transmission by five thrips vector species (Thysanoptera: Thripidae) of tospoviruses in Japan. Appl Entomol Zool 39:71–78

    Article  Google Scholar 

  32. Salomone A, Mongelli M, Roggero P, Boscia D (2004) Reliability of detection of Citrus tristeza virus by an immunochromatographic lateral flow assay in comparison with ELISA. J Plant Pathol 86:43–48

    CAS  Google Scholar 

  33. Schwartz HF, Brown WM, Blunt T, Gent DH (2002) Iris yellow spot virus on onion in Colorado. Plant Dis 86:560

    Article  Google Scholar 

  34. Sin S-H, McNulty BC, Kennedy GG, Moyer JW (2005) Viral genetic determinants for thrips transmission of tomato spotted wilt virus. Proc Natl Acad Sci USA 102:5168–5173

    Article  PubMed  CAS  Google Scholar 

  35. Soellick TR, Uhrig JF, Bucher GL, Kellmann JW, Schreier PH (2000) The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proc Natl Acad Sci USA 70:2373–2378

    Article  Google Scholar 

  36. Storms MMH, Kormelink R, Peters D, Van Lent JWM, Goldbach RW (1995) The nonstructural protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. Virology 214:485–493

    Article  PubMed  CAS  Google Scholar 

  37. Strimmer K, von Heaselen A (1996) Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969

    CAS  Google Scholar 

  38. Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okumo T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532:75–79

    Article  PubMed  CAS  Google Scholar 

  39. Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  40. Turina M, Ciuffo M, Lenzi R, Rostagno L, Mela E, Palmano S (2006) Characterization of four species beloging to the family Potyviridae isolated from Ranunculus asiaticus. Phytopathology 96:560–566

    Article  PubMed  CAS  Google Scholar 

  41. Turina M, Desvoyes B, Sholthof K-BG (2000) A gene cluster encoded by Panicum mosaic virus is associated with virus movement. Virology 266:12–128

    Article  Google Scholar 

  42. Turina M, Maruoke M, Monis J, Jackson AO, Scholthof K-BG (1998) Nucleotide sequence and infectivity of a full-length cDNA clone of Panicum mosaic virus. Virology 241:141–155

    Article  PubMed  CAS  Google Scholar 

  43. Turina M, Ricker MD, Lenzi R, Masenga V, Ciuffo M (2007) A severe disease of tomato in the Culiacan Area (Sinaloa, Mexico) is caused by a new picorna-like viral species. Plant Dis 91:932–941

    Article  CAS  Google Scholar 

  44. Vaira AM, Roggero P, Luisoni E, Masenga V, Milne RG, Lisa V (1993) Characterization of two tospoviruses in Italy: tomato spotted wilt and impatiens necrotic spot. Plant Pathol 42:530–542

    Article  CAS  Google Scholar 

  45. Whitfield AE, Ullman DE, German TL (2005) Tospovirus–thrips interactions. Annu Rev Phytopathol 43:459–489

    Article  PubMed  CAS  Google Scholar 

  46. Winter S, Shahraeen N, Koerbler M, Lesemann D-E (2006) Characterization of Tomato fruit yellow ring virus: a new Tospovirus species infecting tomato in Iran. Plant Pathol 55:287

    Article  Google Scholar 

  47. Zucker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415

    Article  Google Scholar 

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Acknowledgments

Particular thanks to Riccardo Lenzi and Caterina Perrone for technical assistance, to Dr. Marco Pansa for helping with field surveys and to Dr. Robert Milne for carefully editing the manuscript.

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Authors and Affiliations

  1. Istituto di Virologia Vegetale, Sez. di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy

    M. Ciuffo, D. Pacifico, V. Masenga & M. Turina

  2. Di.Va.P.R.A. Entomologia e Zoologia applicate all’Ambiente, University of Torino, Via L. da Vinci 44, 10095, Grugliasco (TO), Italy

    L. Tavella

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  1. M. Ciuffo
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  2. L. Tavella
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  3. D. Pacifico
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Correspondence to M. Turina.

Electronic supplementary material

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705_2008_228_MOESM1_ESM.pdf

Fig. 1: A tomato plant remained PolRSV negative althoughcompletely surrounded by PolRSV infected Polygonum convolvolus (PDF 1387 kb)

Table 1: Primers used for cloning and sequencing of PolRSV smalland medium genome segments (PDF 5 kb)

705_2008_228_MOESM3_ESM.pdf

Table II: DAS-ELISA results for the detection of PolRSV, IYSV and TYRV in weed and vegetable crop samplesfrom 5 lcations in Piedmonta (PDF 9 kb)

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Ciuffo, M., Tavella, L., Pacifico, D. et al. A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus) . Arch Virol 153, 2059–2068 (2008). https://doi.org/10.1007/s00705-008-0228-1

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  • DOI: https://doi.org/10.1007/s00705-008-0228-1

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