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Cowpea golden mosaic disease in Gujarat is caused by a Mungbean yellow mosaic India virus isolate with a DNA B variant

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Abstract

It has long been assumed that cowpea golden mosaic disease (CGMD) in southern Asia is caused by a begomovirus distinct from those causing disease in other legumes. The components of a begomovirus causing CGMD in western India were isolated, cloned and sequenced. Analysis of the sequences shows the virus to be an isolate of Mungbean yellow mosaic India virus, but with a distinct DNA B component with greater similarity to components of a second legume-infecting begomovirus occurring in the region, Mungbean yellow mosaic virus. The clones of the virus were readily infectious to cowpea, mungbean, blackgram and French bean by agroinoculation. However, the wild-type isolate was shown to be easily transmissible by whiteflies between cowpea plants but not to blackgram and mugbean, suggesting that the insect vector plays a major role in determining the natural host range of these viruses.

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Acknowledgments

We thank, Dr. S.A. Patil, Director, Indian Agricultural Research Institute for providing the facilities. Priya John is thankful to Indian Council of Agricultural Research for the Fellowship. This study was supported by a grant from the Department of Biotechnology (BT/PR/1834, AGR/07/86/99), Government of India.

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Author notes

  1. N. Kumar & A. Mishra

    Present address: MAHYCO Seeds Ltd, Jalna, Aurangabad Road, P.O. Box 76, Dawaldi, Jalna, 431203, Maharashtra, India

Authors and Affiliations

  1. Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    P. John, P. N. Sivalingam, Q. M. I. Haq, N. Kumar, A. Mishra & V. G. Malathi

  2. National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan

    R. W. Briddon

  3. Jain Irrigation System Limited, Jain Hills, Agri Park, Shirsoli Road, Jalgaon, 425001, Maharashtra, India

    A. Mishra

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  1. P. John
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Correspondence to V. G. Malathi.

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John, P., Sivalingam, P.N., Haq, Q.M.I. et al. Cowpea golden mosaic disease in Gujarat is caused by a Mungbean yellow mosaic India virus isolate with a DNA B variant. Arch Virol 153, 1359–1365 (2008). https://doi.org/10.1007/s00705-008-0116-8

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