Skip to main content

Advertisement

SpringerLink
Log in

Comparison of four infectious bursal disease viruses isolated from different bird species

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Four isolates of infectious bursal disease virus (IBDV), isolated from chicken, duck, goose and sparrow in Jiangsu province of China in 2002, were compared. The viruses were stable to the treatments of 60 °C for 1 h, pH 2.0 and lipid solvents. Their antigenic relatedness values (R) were from 0.76 to 0.78. Chickens infected with the chicken isolate showed severe clinical symptoms of IBD and the mortality rate was 33.3% (2/6). Chickens infected with the other three viruses survived but their bursas were damaged and the bursa/body-weight ratios were lower than those of the uninfected control (p< 0.01). The titers of anti-IBDV antibody in infected chicken sera reached up to 1600 by virus neutralization and 6400 by ELISA at 10 days post infection. The sequences of the variable region of VP2 were aligned and compared, showing nucleotide variations ranging from 1.5 to 6.7% and deduced aminoacid variations from 0.8 to 2.2%. All had the same heptapeptide, S-W-S-A-S-G-S, Asp279, and Ala284. The four viruses clustered on a phylogenetic tree and were distant from the STC strain. These findings suggested that different bird species naturally infected with IBDV could serve as carriers or reservoirs in IBDV transmission and might play a role in the emergence of variant IBDV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

aa:

amino acid

BB ratio:

bursa weight in grams × 1000/total body weight in grams

CEF:

chicken embryo fibroblast

Ch:

IBDV isolated from chicken

CPE:

cytopathic effects

Du:

IBDV isolated from duck

Go:

IBDV isolated from goose

IBD(V):

infectious bursal disease (virus)

MAb:

monoclonal antibody

nt:

nucleotide

Sp:

IBDV isolated from sparrow

SPF:

specific-pathogen-free

References

  • AA Azad MN Jagadish MN Brown PJ Hudson (1987) ArticleTitleDeletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus Virology 161 145–152 Occurrence Handle2445101 Occurrence Handle10.1016/0042-6822(87)90180-2 Occurrence Handle1:CAS:528:DyaL1cXhsFak

    Article  PubMed  CAS  Google Scholar 

  • Baxendale W (1976) The development of an apathogenic infectious bursal agent vaccine: field trial results. Proc. 25th Western Poultry Disease Conference and 10th Poultry Health Symposium. University of California, Davis, USA

  • CD Bayliss U Spies K Shaw RW Peters A Pagageorgiou H Müller MEG Boursnell (1990) ArticleTitleA comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region of VP2 J Gen Virol 71 1303–1312 Occurrence Handle2161902 Occurrence Handle1:CAS:528:DyaK3cXlvVGnurc%3D

    PubMed  CAS  Google Scholar 

  • H Becht H Muller HK Muller (1988) ArticleTitleComparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus J Gen Virol 69 631–640 Occurrence Handle2450966 Occurrence Handle1:CAS:528:DyaL1cXhsVyms7o%3D

    PubMed  CAS  Google Scholar 

  • WJ Benton MS Cover JK Rosenberger RS Lake (1967) ArticleTitlePhysicochemical properties of the infectious bursal agent (IBA) Avian Dis 11 438–445 Occurrence Handle6052471 Occurrence Handle10.2307/1588192 Occurrence Handle1:CAS:528:DyaF2sXkvVyntr4%3D

    Article  PubMed  CAS  Google Scholar 

  • HJ Boot AJ Hoekman AL Gielkens (2005) ArticleTitleThe enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment Arch Virol 150 137–144 Occurrence Handle15654506 Occurrence Handle10.1007/s00705-004-0405-9 Occurrence Handle1:CAS:528:DC%2BD2MXksVKisQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • HJ Boot AA Huurne AJ Hoekman BP Peeters AL Gielkens (2000) ArticleTitleRescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulentphenotype J Virol 74 6701–6711 Occurrence Handle10888607 Occurrence Handle10.1128/JVI.74.15.6701-6711.2000 Occurrence Handle1:CAS:528:DC%2BD3cXkvFCmsbY%3D

    Article  PubMed  CAS  Google Scholar 

  • M Brandt K Yao M Liu RA Heckert VN Vakharia (2001) ArticleTitleMolecular determinants of virulence, cell tropism, and pathogenic phenotype of infectious bursal disease virus J Virol 75 11974–11982 Occurrence Handle11711587 Occurrence Handle10.1128/JVI.75.24.11974-11982.2001 Occurrence Handle1:CAS:528:DC%2BD3MXovVygsLc%3D

    Article  PubMed  CAS  Google Scholar 

  • MD Brown P Green MA Skinner (1994) ArticleTitleVP2 sequences of recent European “ very virulent” isolates of infectious bursal disease virus are closely related to each other but are distinct from those of “ classical” strains J Gen Virol 75 675–680 Occurrence Handle8126466 Occurrence Handle1:CAS:528:DyaK2cXktVaitbs%3D

    PubMed  CAS  Google Scholar 

  • YC Cao YZ Bi FC Leung BL Lim (1998) ArticleTitleMolecular signature of host-protective antigen of highly virulent strains of infectious bursal disease virus Chin J Vet Sci 18 521–526 Occurrence Handle1:CAS:528:DyaK1MXht12ntr0%3D

    CAS  Google Scholar 

  • YC Cao WS Yeung M Law YZ Bi FC Leung BL Lim (1998) ArticleTitleMolecular characterization of seven Chinese isolates of infectious bursal disease virus: classical, very virulent and variant strains Avian Dis 42 340–351 Occurrence Handle9645325 Occurrence Handle10.2307/1592484 Occurrence Handle1:STN:280:DyaK1czgvFajtQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • ZZ Cui SH Sun ZF Shan YW Jiang WJ Jin (2002) ArticleTitlePathogenicity of a very virulent strain GX8/99 of infectious bursal disease virus Chin J Virol 18 162–166

    Google Scholar 

  • SA Edgar Y Cho (1965) ArticleTitleAvian nephrosis (Gumboro disease) and its control by immunization Poultry Sci 44 1366

    Google Scholar 

  • KJ Fahey K Erny J Crooks (1989) ArticleTitleA conformational immunogen on VP2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens J Gen Virol 70 1473–1481 Occurrence Handle2543787 Occurrence Handle1:CAS:528:DyaL1MXktlOmtLw%3D

    PubMed  CAS  Google Scholar 

  • Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2005) Virus taxonomy. Classification and nomenclature of viruses. 8th ICTV Report. Academic Press, Elsevier, San Diego, pp 566–567

  • BT Grenfell OG Pybus JR Gog JL Wood JM Daly JA Mumford EC Holmes (2004) ArticleTitleUnifying the epidemiological and evolutionary dynamics of pathogens Science 303 327–332 Occurrence Handle14726583 Occurrence Handle10.1126/science.1090727 Occurrence Handle1:CAS:528:DC%2BD2cXjtFGmuw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • HG Heine M Haritou P Failla K Fahey A Azad (1991) ArticleTitleSequence analysis and expression of the host-protective immunogen VP2 of a variant strain of infectious bursal disease virus which can circumvent vaccination with standard type 1 strains J Gen Virol 72 1835–1843 Occurrence Handle1651980 Occurrence Handle1:CAS:528:DyaK3MXltlelsrc%3D

    PubMed  CAS  Google Scholar 

  • CC Hon TY Lam A Drummond A Rambaut YF Lee CW Yip F Zeng PY Lam PT Ng FC Leung (2006) ArticleTitlePhylogenetic analysis reveals a correlation between the expansion of very virulent infectious bursal disease virus and reassortment of its genome segment B J Virol 80 8503–8509 Occurrence Handle16912300 Occurrence Handle10.1128/JVI.00585-06 Occurrence Handle1:CAS:528:DC%2BD28XovVOjt7s%3D

    Article  PubMed  CAS  Google Scholar 

  • RI Howie J Thorsen (1981) ArticleTitleIdentification of a strain of infectious bursal disease virus isolated from mosquitoes Can J Comp Med 45 315–320 Occurrence Handle6280817 Occurrence Handle1:STN:280:Bi2C1czosV0%3D

    PubMed  CAS  Google Scholar 

  • Z Huang S Elankumaran AS Yunus SK Samal (2004) ArticleTitleA recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV J Virol 78 10054–10063 Occurrence Handle15331738 Occurrence Handle10.1128/JVI.78.18.10054-10063.2004 Occurrence Handle1:CAS:528:DC%2BD2cXnvVarsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • PJ Hudson NM Mckern BE Power AA Azad (1986) ArticleTitleGenomic structure of the large RNA segment of infectious bursal disease virus Nucleic Acids Res 14 5001–5012 Occurrence Handle3014441 Occurrence Handle10.1093/nar/14.12.5001 Occurrence Handle1:CAS:528:DyaL28XkvFahsr8%3D

    Article  PubMed  CAS  Google Scholar 

  • MR Islam K Zierenberg H Muller (2001) ArticleTitleThe genome segment B encoding the RNA-dependent RNA polymerase protein VP1 of very virulent infectious bursal disease virus (IBDV) is phylogenetically distinct from that of all other IBDV strains Arch Virol 146 2481–2492 Occurrence Handle11811695 Occurrence Handle10.1007/s007050170018 Occurrence Handle1:CAS:528:DC%2BD38Xjs12ksb8%3D

    Article  PubMed  CAS  Google Scholar 

  • NM Ismail YM Saif PD Moorhead (1988) ArticleTitleLack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens Avian Dis 32 757–759 Occurrence Handle2849404 Occurrence Handle10.2307/1590995 Occurrence Handle1:STN:280:BiaD2snhtVM%3D

    Article  PubMed  CAS  Google Scholar 

  • DH Jackwood YM Saif (1987) ArticleTitleAntigenic diversity of infectious bursal disease viruses Avian Dis 31 766–770 Occurrence Handle2831868 Occurrence Handle10.2307/1591028 Occurrence Handle1:STN:280:BieC283ntFE%3D

    Article  PubMed  CAS  Google Scholar 

  • DH Jackwood YM Saif JH Hughes (1987) ArticleTitleReplication of infectious busal disease virus in continuous cell lines Avian Dis 31 370–375 Occurrence Handle3039967 Occurrence Handle10.2307/1590888 Occurrence Handle1:STN:280:BiiB1Mfjt1Y%3D

    Article  PubMed  CAS  Google Scholar 

  • DJ Jackwood YM Saif JH Hughes (1982) ArticleTitleCharacteristics and serologic studies of two serotypes of infectious bursal disease virus in turkeys Avian Dis 26 871–882 Occurrence Handle6297451 Occurrence Handle10.2307/1589875 Occurrence Handle1:STN:280:BiyC38njsFE%3D

    Article  PubMed  CAS  Google Scholar 

  • FSB Kibenge DJ Jackwood CC Mercado (1990) ArticleTitleNucleotide sequence analysis of genome segment A of infectious bursal disease virus J Gen Virol 71 569–577 Occurrence Handle2155995 Occurrence Handle1:CAS:528:DyaK3cXlsVKit7Y%3D

    PubMed  CAS  Google Scholar 

  • HN Lasher SM Shane (1994) ArticleTitleInfectious bursal disease Worlds Poult Sci J 50 133–166 Occurrence Handle10.1079/WPS19940013

    Article  Google Scholar 

  • C Le Nouen G Rivallan D Toquin P Darlu Y Morin V Beven C de Boisseson C Cazaban S Comte Y Gardin N Eterradossi (2006) ArticleTitleVery virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate J Gen Virol 87 209–216 Occurrence Handle16361433 Occurrence Handle10.1099/vir.0.81184-0 Occurrence Handle1:CAS:528:DC%2BD28XktlGhtg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • BL Lim Y Cao T Yu CW Mo (1999) ArticleTitleAdaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2 J Virol 73 2854–2862 Occurrence Handle10074133 Occurrence Handle1:CAS:528:DyaK1MXhvFynsLc%3D

    PubMed  CAS  Google Scholar 

  • M Liu VN Vakharia (2004) ArticleTitleVP1 protein of infectious bursal disease virus modulates the virulence in vivo Virology 330 62–73 Occurrence Handle15527834 Occurrence Handle10.1016/j.virol.2004.09.009 Occurrence Handle1:CAS:528:DC%2BD2cXpsFentbg%3D

    Article  PubMed  CAS  Google Scholar 

  • PD Lukert RB Davis (1974) ArticleTitleInfectious bursal disease virus: growth and characterization in cell cultures Avian Dis 18 243–250 Occurrence Handle4364080 Occurrence Handle10.2307/1589133 Occurrence Handle1:CAS:528:DyaE2cXkslCksLo%3D

    Article  PubMed  CAS  Google Scholar 

  • PD Lukert YM Saif (1997) Infectious bursal disease BW Calnek (Eds) Diseases of poultry EditionNumber10 Iowa State University Press Ames 721–738

    Google Scholar 

  • JB McFerran MS McNulty ER McKillop TJ Conner RM McCracken DS Collins GM Allan (1980) ArticleTitleIsolation and serological studies with infectious bursal disease viruses from fowl, turkey and duck: demonstration of a second serotype Avian Pathol 9 395–404

    Google Scholar 

  • H Muller MR Islam R Raue (2003) ArticleTitleResearch on infectious bursal disease-the past, the present and the future Vet Microbiol 97 153–165 Occurrence Handle14637046 Occurrence Handle10.1016/j.vetmic.2003.08.005

    Article  PubMed  Google Scholar 

  • H Muller R Nitschke (1987) ArticleTitleThe two segments of the infectious bursal disease virus genome are circularized by a 90000-Da protein Virology 159 174–177 Occurrence Handle3037777 Occurrence Handle10.1016/0042-6822(87)90363-1 Occurrence Handle1:STN:280:BiiB28rks1M%3D

    Article  PubMed  CAS  Google Scholar 

  • E Mundt J Beyer H Muller (1995) ArticleTitleIdentification of a novel viral protein in infectious bursal disease virus-infected cells J Gen Virol 76 437–443 Occurrence Handle7844565 Occurrence Handle1:CAS:528:DyaK2MXjtl2jtrg%3D Occurrence Handle10.1099/0022-1317-76-2-437

    Article  PubMed  CAS  Google Scholar 

  • E Mundt B Kollner D Kretzschmar (1997) ArticleTitleVP5 of infectious bursal disease virus is not essential for virus replication in cell culture J Virol 71 5647–5651 Occurrence Handle9188642 Occurrence Handle1:CAS:528:DyaK2sXjvVKrtL4%3D

    PubMed  CAS  Google Scholar 

  • MM Nagarajan FSB Kibenge (1997) ArticleTitleInfectious bursal disease virus: a review of molecular basis for variations in antigenicity and virulence Can J Vet Res 61 81–88 Occurrence Handle9114957 Occurrence Handle1:CAS:528:DyaK2sXivFGku7o%3D

    PubMed  CAS  Google Scholar 

  • SV Nikolakaki K Nomikou M Koumbati O Mangana M Papanastassopoulou PP Mertens O Papadopoulos (2005) ArticleTitleMolecular analysis of the NS3/NS3A gene of Bluetongue virus isolates from the 1979 and 1998– 2001 epizootics in Greece and their segregation into two distinct groups Virus Res 114 6–14 Occurrence Handle15993974 Occurrence Handle10.1016/j.virusres.2005.05.004 Occurrence Handle1:CAS:528:DC%2BD2MXht1WjtLfE

    Article  PubMed  CAS  Google Scholar 

  • S Ohashi Y Matsumori T Yanase M Yamakawa T Kato T Tsuda (2004) ArticleTitleEvidence of an antigenic shift among Palyam serogroup orbiviruses J Clin Microbiol 42 4610–4614 Occurrence Handle15472317 Occurrence Handle10.1128/JCM.42.10.4610-4614.2004 Occurrence Handle1:CAS:528:DC%2BD2cXpvFKquro%3D

    Article  PubMed  CAS  Google Scholar 

  • RF Ramig (1997) ArticleTitleGenetics of the rotaviruses Annu Rev Microbiol 51 225–255 Occurrence Handle9343350 Occurrence Handle10.1146/annurev.micro.51.1.225 Occurrence Handle1:CAS:528:DyaK2sXms1Shsbk%3D

    Article  PubMed  CAS  Google Scholar 

  • Rosenberger JK, Cloud SS (1986) Isolation and characterization of variant infectious bursal disease viruses (Abstract no. 181). Proc. 123rd Annual Meeting of the American Veterinary Medicine Association

  • CJ Russell RG Webster (2005) ArticleTitleThe genesis of a pandemic influenza virus Cell 123 368–371 Occurrence Handle16269328 Occurrence Handle10.1016/j.cell.2005.10.019 Occurrence Handle1:CAS:528:DC%2BD2MXht1altLjE

    Article  PubMed  CAS  Google Scholar 

  • DB Snyder DP Lana PK Savage FS Yancey SA Mengel WW Marquardt (1988) ArticleTitleDifferentiation of infectious bursal disease viruses directly from infected tissues with neutralizing monoclonal antibodies: evidence of a major antigenic shift in recent field isolates Avian Dis 32 535–539 Occurrence Handle2461698 Occurrence Handle10.2307/1590924 Occurrence Handle1:STN:280:BiaD287ls10%3D

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673– 4680. www.ebi.ac.uk/clustalw/index.html

  • VN Vakharia J He B Ahamed DB Snyder (1994) ArticleTitleMolecular basis of antigenic variation in infectious bursal disease virus Virus Res 31 265–273 Occurrence Handle8178574 Occurrence Handle10.1016/0168-1702(94)90009-4 Occurrence Handle1:CAS:528:DyaK2cXivFagsr4%3D

    Article  PubMed  CAS  Google Scholar 

  • YS Wang HJ Fan Y Li ZL Shi Y Pan CP Lu (2007) ArticleTitleDevelopment of a multi-mimotope peptide as a vaccine immunogen for infectious bursal disease virus Vaccine 25 4447–4455 Occurrence Handle17445956 Occurrence Handle10.1016/j.vaccine.2007.03.018 Occurrence Handle1:CAS:528:DC%2BD2sXkvFGksrg%3D

    Article  PubMed  CAS  Google Scholar 

  • YS Wang ZA Zhou Y Liu JB Hou KW He C Ding XZ Deng J Gao ZY Diao (2003) ArticleTitleIsolation of infectious bursal disease virus from different bird species and analysis of their biological properties Prog Vet Med 24 84–86

    Google Scholar 

  • YS Wang ZA Zhou CS Zhai HL Luo C Ding Y Fang (1997) ArticleTitleStudy on the ecological surveys on non-chicken avian species for natural infections by IBDV Chin J Vet Sci Tech 27 15–16

    Google Scholar 

  • YS Wang ZA Zhou CS Zhai YL Wang Y Fang ZX Gu (1994) ArticleTitleThe use of DIG-Labelled cDNA probe for the identification of infectious bursal disease virus Chin J Vet Sci 14 151–153

    Google Scholar 

  • T Yamaguchi M Ogawa Y Inoshima M Miyoshi H Fukushi K Hirai (1996) ArticleTitleIdentification of sequence changes responsible for the attenuation of highly virulent infectious bursal disease virus Virology 223 219–223 Occurrence Handle8806555 Occurrence Handle10.1006/viro.1996.0470 Occurrence Handle1:CAS:528:DyaK28XlsVemt7s%3D

    Article  PubMed  CAS  Google Scholar 

  • T Yamaguchi M Ogawa M Miyoshi Y Inoshima H Fukushi K Hirai (1997) ArticleTitleSequence and phylogenetic analyses of highly virulent infectious bursal disease virus Arch Virol 142 1441–1458 Occurrence Handle9267454 Occurrence Handle10.1007/s007050050171 Occurrence Handle1:CAS:528:DyaK2sXls1ymtrY%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Disease Control and Prevention of Nanjing Command, Huadong Research Institute of Medical Biotechnics, Nanjing, China

    Y. S. Wang, Z. C. Wang, Y. D. Tang & Z. L. Shi

  2. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China

    Y. S. Wang, K. W. He, Y. Li & J. B. Hou

  3. Department of Veterinary Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China

    Y. S. Wang, H. C. Yao, H. J. Fan & C. P. Lu

Authors
  1. Y. S. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. C. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. D. Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z. L. Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. W. He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. B. Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. H. C. Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. H. J. Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. C. P. Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., Wang, Z., Tang, Y. et al. Comparison of four infectious bursal disease viruses isolated from different bird species. Arch Virol 152, 1787–1797 (2007). https://doi.org/10.1007/s00705-007-1022-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-1022-1

Keywords

Search

Navigation