Summary.
We described the construction of a recombinant filamentous phage displaying on its surface the immunodominant site of VP1 protein of foot-and-mouth disease virus (FMDV). The coding sequence was inserted at the amino-terminus of the major coat protein pVIII via a spacer. The hybrid phage proved to be antigenic as it was recognized by polyclonal and monoclonal anti FMDV sera. In two experiments involving immunisation of guinea-pigs with the recombinant phage, a low antibody response was generated. This suggests a possible role for phage displayed peptides in inducing anti FMDV immunity and the possibility of further development is discussed.
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Kim, Y., Lebreton, F., Kaiser, C. et al. Expression of a Foot-and-mouth disease virus immunodominant epitope by a filamentous bacteriophage vector. Arch Virol 149, 365–377 (2004). https://doi.org/10.1007/s00705-003-0192-8
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DOI: https://doi.org/10.1007/s00705-003-0192-8