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A new species of Scytonema isolated from Bilaspur, Chhattisgarh, India using the polyphasic approach

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Abstract

A false branching cyanobacterium (strain 1F-PS) isolated from a fresh water body of Bilaspur (Chhattisgarh, India) is described here as a new species of the polyphyletic genus Scytonema. Morphological, ecological, molecular and phylogenetic evidence validated the strain as a new species. Observations of the filaments in different phases of growth, different levels of microscopic studies, the presence of a textured thin sheath throughout the length of the trichome, differences in the shape and dimensions of the vegetative cells and heterocytes and ecological attributes show that the strain differed from the rest of the closely related species. Sequencing of the 16S rRNA gene resulted in 99% similarity with Scytonema bilaspurensis and 97.07% sequence similarity with Scytonema hofmannii PCC 7110, while rbcl and psbA sequences showed 99 and 97% similarities with S. bilaspurensis, respectively. Phylogenetic assessments indicated a large phylogenetic distance and separate clustering of the strain 1F-PS for all the molecular markers.

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Acknowledgements

We are thankful to the Council of Scientific and Industrial Research, New Delhi, India for financial support in the form of project (Ref. No. 38(1333)/12/EMR(II). The Head Department of Botany, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India is gratefully acknowledged for providing laboratory facilities. We thank the Director NCCS for facilities and encouragement. Authors from NCCS are thankful to the Department of Biotechnology (DBT; Grant no. BT/PR/0054/NDB/52/94/2007), the Government of India, under the project ‘Establishment of Microbial Culture Collection.’

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Authors and Affiliations

  1. Department of Botany, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, 495009, India

    Robin A. Minz, Kikku Kunui & Satya S. Singh

  2. Microbial Culture Collection (MCC), National Centre for Cell Science (NCCS), Pune, India

    Prashant Singh, Zaid M. Shaikh, Archana Suradkar & Yogesh S. Shouche

  3. Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Arun K. Mishra

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  1. Prashant Singh
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  2. Robin A. Minz
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  3. Kikku Kunui
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  4. Zaid M. Shaikh
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  8. Satya S. Singh
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Correspondence to Satya S. Singh.

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Handling editor: Ricarda Riina.

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Information on Electronic Supplementary Material

Online Resource 1 . 16S rRNA alignment file with the strain 1F-PS and all the considered taxa.

Online Resource 2 . rbcl alignment file with the strain 1F-PS and all the considered taxa.

Online Resource 3 . psbA alignment file with the strain 1F-PS and all the considered taxa.

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Singh, P., Minz, R.A., Kunui, K. et al. A new species of Scytonema isolated from Bilaspur, Chhattisgarh, India using the polyphasic approach. Plant Syst Evol 303, 249–258 (2017). https://doi.org/10.1007/s00606-016-1370-y

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