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Proof of a knowledge database concept. Aubrieta ekimii (Brassicaceae), a new species from NW Anatolia (Turkey): morphological and molecular support

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Abstract

Aubrieta ekimii, a new species from Kocaeli Province (Turkey), is described and illustrated, and its relationship to putatively closest relatives, A. olympica and A. pinardii, is discussed. Scanning electron microscopy micrographs of the indumentum of A. ekimii and its relatives are presented. Cytology, ecology, conservation status, and geographical distribution of the novelty and related species are presented. The knowledge database BrassiBase (http://brassibase.cos.uni-heidelberg.de/index.php) is shown herein as a powerful tool to recognize potentially new species. DNA sequence data from the transcribed spacers of nuclear encoded ribosomal RNA (ITS1 and ITS2) was subjected to the respective phylogenetic placement algorithm in BrassiBase and, after adding further sequence information from the plastid trnLF region, tribal-wide phylogenetic analyses were conducted to confirm the systematic placement of the novelty. Because of limited DNA sequence variation, we did not obtain a highly resolved phylogenetic hypothesis of Aubrieta. Our study further highlights Anatolia as a craddle of species diversity with many overlooked and undescribed species.

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Acknowledgments

We are grateful to the curators of ANK, BULU, GAZI, HUB and ISTO for allowing access to their Aubrieta material. Our thanks go also to Dr. Özer Yılmaz for his assistance during field trips in Mt. Uludağ and funding for the research from “Fund of İstanbul University (project no. 31628, 43913)” was highly appreciated by S.Y. Research of M.A.K. was supported by several grants from DFG (Deutsche Forschungsgemeinschaft): KO2302/12-1; KO2302/13-1 and 13-2, and that of I.A.S. by the US National Science Foundation grant DEB-1252905.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Beyazıt, 34116, Istanbul, Turkey

    Sırrı Yüzbaşıoğlu

  2. Department of Biodiversity and Plant Systematics, Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, 69120, Heidelberg, Germany

    Marcus A. Koch

  3. Missouri Botanical Garden, St. Louis, 63166-0299, USA

    Ihsan I. A. Al-Shehbaz

Authors
  1. Sırrı Yüzbaşıoğlu
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  2. Marcus A. Koch
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Correspondence to Sırrı Yüzbaşıoğlu.

Additional information

Handling editor: Karol Marhold.

Electronic supplementary material

Below is the link to the electronic supplementary material.

606_2015_1212_MOESM1_ESM.txt

Online resource Figs. S1 and S2: ITS and trnLF alignments of 313 taxa from tribe Arabideae including the genus Aubrieta. (TXT 218 kb)

606_2015_1212_MOESM2_ESM.txt

Online resource Figs. S3 and S4: Reduced ITS and trnLF alignments, comprising all Aubrieta sequences plus three additional outgroup taxa. (TXT 397 kb)

Supplementary material 3 (TXT 15 kb)

Supplementary material 4 (TXT 26 kb)

Appendix 1

Appendix 1

List of Specimens examined and according information about the vouchers.

Aubrieta ekimii (paratypes): Turkey, A2(A) Kocaeli, Yuvacık, Soğuksu, Menekşe yaylası patikası, Şahin kaya, 870 m a. s. l., kaya üstü, 30 May 2009, S. Yüzbaşıoğlu 3207 (ISTE 106646!); ibid., 1000 m a. s. l., 17 Apr 2010, N. Aksoy 6766 (DUOF 5842!); ibid., 27 May 2010, S. Yüzbaşıoğlu 3222 (ISTE 106647!); Serindere, vadi içi, 340 m a. s. l., 20 Apr 2014, S. Yüzbaşıoğlu 3972 (ISTE 106648!); ibid., 19 May 2014, S. Yüzbaşıoğlu 3991 (ISTE 106649!); ibid., 11 Jul 2014, S. Yüzbaşıoğlu 4100 (ISTE 106650!), İ. Genç, F. Canız, ibid., 314 m a. s. l., 17 Apr 2010, N. Aksoy 6758 (DUOF 5841!).

Aubrieta olympica Boiss.: —Turkey. A2(A) Bursa: Uludağ, 2400 m a. s. l., 13 Sep 1947, Davis 14813 (ANK!); Uludağ, summit, northern slopes, 2300 m a. s. l., 25 Jun 1980, A.J. Byfield 1127, R. Fitzgerald s.n. (ISTE 67249!); Uludağ, Kaplıkaya, in the valley, 250 m a. s. l., 17 May 1984, B. Tözün et al. (BULU 20!); Soğukpınar, Aras valley, rocky slopes, 1300–1700 m a. s. l., 12 Jul 2001, G.Kaynak, A. Bıçakcı, G. Güleryüz, Ö. Yılmaz s.n. (BULU 12518!); ibid., 1550 m a. s. l., 06 Jun 2002, G. Kaynak, R. Günay, Ö. Yılmaz s.n. (BULU 14086!); ibid., 06 Jun 2002, G. Kaynak, R. Günay, Ö. Yılmaz s.n. (BULU 14087!); ibid., 1400–2080 m a. s. l., 16 Jun 2004, R. Daşkın, Ö. Yılmaz, E. Erdoğan, T. Cenil s.n. (BULU 19779!); ibid., 1490 m a. s. l., 07 Jul 2014, S. Yüzbaşıoğlu 4074, Ö. Yılmaz, İ. Genç, F. Canız s.n. (ISTE 106681!); Paşaçayırı–Kuşaklıkaya, rocky places, 2000–2100 m a. s. l., G. Kaynak, R. Daşkın, Ö. Yılmaz, E. Erdoğan s.n. (BULU 19990!); Keles, Sorgun–Tunçbilek road junction, Tunçbilek road, slopes, 910–950 m a. s. l., 17 Apr 2003, G. Kaynak, R. Daşkın, Ö. Yılmaz s.n. (BULU 14770!); Bursa–Keles road, Soğukpınar–Karaıslah road junction, 850–900 m a. s. l., 27 May 2003, G. Kaynak, R. Günay, Ö. Yılmaz s.n.(BULU 15362!); above Bozova plateau, Çavuşdüzü, rocky places, 1900–2120 m a. s. l., 09 Jul 2003, G. Kaynak, H. Malyer, R. Günay, Ö. Yılmaz s.n. (BULU 17545!); Mustafakemalpaşa, Ayva village, moist rocky places, 19 Apr 2009, G. Kaynak s.n. (BULU 30916!); Alaçam–Göller region, 23 Jul 2012, G. Kaynak, Ö. Yılmaz, G. Bağcıvan s.n. (BULU 34410!); ibid. 2215 m a. s. l., 07 Jul 2014, S. Yüzbaşıoğlu 4075, Ö. Yılmaz, İ. Genç, F. Canız s.n. (ISTE 106681!).

Aubrieta pinardii Boiss.:—Turkey. B2 Denizli: Akdağ, east of Çivril, rocky slopes, 26 May 1991, J. Zielinski s.n. (ISTE 65961!); B3 Afyon: Sandıklı–Dinar road, 18 km before Dinar, Afşar village, arid hills, 22 May 1966, A. Baytop and B. Çubukçu s.n. (ISTE 9422!); Sandıklı–Şuhut road, Kumalar plateau, 1500 m a. s. l., 18 May 2001, E. Akçiçek 3607 (GAZI!); Konya: Akşehir, Engilli plateau, Gıloğlu stream, on the rocks, 1380 m a. s. l., 31 May 1974, G. Dökmeci and Y. Doğantan s.n. (ISTE 28885a!); Akşehir, Monas village, 30 May 1974, G. Dökmeci and Y. Doğantan s.n. (ISTE 28835!); C3 Isparta: Davras mountain, Işık şamı, 1350 m a. s. l., 30 May 1955, A. and T. Baytop s.n. (ISTE 4271!); Dedegöl mountains, ascent from Yenişarbademli, 2300 m a. s. l., 15 Jul 1999, M. Döring, G. Parolly and D. Tolimir s.n. (ISTE 100556!); Eğirdir, 2 km south of Yaka village, 1800 m a. s. l., 20 May 1973, H. Peşmen and A. Güner 3500 (ANK!); Antalya: Antalya–Burdur road, 1 km before to the border of Burdur, 750 m a. s. l., 21 May 1982, N. and E. Özhatay s.n. (ISTE 48799!); Antalya: 1120 m a. s. l., 13 May 1956, H. Birand 11 (ANK!); Burdur: Antalya–Burdur, around Çeltikçibeli, 1225 m a. s. l., 21 May 1982, N. and E. Özhatay s.n. (ISTE 48809!); Burdur–Çeltikçi, Kaplanpınar, rocky slopes, 21 May 1966, A. Baytop and B. Çubukçu s.n. (ISTE 9519!); Ağlasun, Başköy, 1320 m a. s. l., 29 May 1997, N. Adıgüzel 2796 and H. Özler (GAZI!); Konya: Beyşehir–Şarkikaraağaç, Feleköy-Asarkuzu region, Kotan hill, 13 Jun 1975, A. Çubukçu s.n. (ISTE 34255!); C4 Konya: Karapınar, Akören village, 1335 m a. s. l., 17 May 2002, S. Yüzbaşıoğlu 1709 (ISTE 99433!); Ayrancı dam, 10 km from Ayrancı, stony hills, 1310 m a. s. l., 22 Apr 1976, A. Baytop et al. s.n. (ISTE 34616!); C5 Niğde: Ulukışla, Bolkar mountains, above Meydan, 2620 m a. s. l., 27 Jul 1999, M. Döring, G. Parolly and D. Tolimir s.n. (ISTE 100650!).

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Yüzbaşıoğlu, S., Koch, M.A. & Al-Shehbaz, I.I.A. Proof of a knowledge database concept. Aubrieta ekimii (Brassicaceae), a new species from NW Anatolia (Turkey): morphological and molecular support. Plant Syst Evol 301, 2043–2055 (2015). https://doi.org/10.1007/s00606-015-1212-3

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