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Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis

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Abstract

Dendrobium officinale (Orchidaceae) is used for traditional medicine and is critically endangered in China. To investigate the genetic structure of this species and to offer some advice on conservation strategies, 84 individuals from nine wild populations of D. officinale were analyzed using the method of sequence-related amplified polymorphism. A high level of genetic diversity was detected (PPB = 88.07%, H E = 0.2880) at the species level. However, the genetic diversity at the population level was lower (PPB = 51.68%, H E = 0.1878) in comparison with other species with similar life history characteristics. Based on analysis of molecular variation, there was moderate variation between pairs of populations with Φ ST values ranging from 0.1327 to 0.4151 and on average 27.05% of the genetic variation occurred among populations. Two main clusters were shown in UPGMA using TFPGA, which is consistent with the result of principal coordinate analysis using NTSYS. In situ conservation is the first advocated and and ex situ should be proposed at the same time to protect the endangered plant and to preserve germplasm resources.

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Acknowledgments

The authors thank the National Natural Science Foundation of China (No. 30370144) for financial support. We are also grateful to Prof. Song Ge for his assistance with softwares, Mr. Ye Xu for his help during figure drawing.

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Authors and Affiliations

  1. Jiangsu Provicinal Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, No. 1, Wenyuan Road, Nanjing, 210046, China

    Ge Ding, Xiaoyu Ding, Qi Zhou, Weichao Zhang & Xuexia Li

  2. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers College, Yancheng, Jiangsu, 224002, China

    Daizhen Zhang

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  1. Ge Ding
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  2. Daizhen Zhang
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  3. Xiaoyu Ding
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  4. Qi Zhou
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  5. Weichao Zhang
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  6. Xuexia Li
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Correspondence to Xiaoyu Ding.

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Ge Ding and Daizhen Zhang made equal contribution to this work.

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Ding, G., Zhang, D., Ding, X. et al. Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis. Plant Syst Evol 276, 149–156 (2008). https://doi.org/10.1007/s00606-008-0068-1

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